High-purity DNA extraction from animal tissue using picking in the TRIzol-based method

TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the picking method's DNA is ideal and...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:	BioTechniques. - 1988. - 70(2021), 3 vom: 18. März, Seite 186-190
1. Verfasser:	Bo, Ye Yin (VerfasserIn)
Weitere Verfasser:	Liang, Li Dong, Hua, Yang Jiang, Zhao, Zhang, Yao, Mei Si, Shan, Liu Bao, Liang, Chen Ze
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2021
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DNA TRIzol classic method extraction facilitate high purity molecular biology assays picking mehr... viral DNA Guanidines Phenols Proteins trizol 9007-49-2

Beschreibung
Zusammenfassung:	TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the picking method's DNA is ideal and better than that obtained by the classic TRIzol method. Gel electrophoresis showed that there was no RNA contamination, and the DNA had not degraded. DNA extracted by the picking method had the same performance in restriction enzyme digestion and quantitative PCR as that obtained by the traditional method. Viral DNA in the infected tissue was also obtained. This modified method facilitates various molecular biology assays
Beschreibung:	Date Completed 06.12.2021 Date Revised 14.12.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1940-9818
DOI:	10.2144/btn-2020-0142