Assembly of Multi-Spheroid Cellular Architectures by Programmable Droplet Merging

Assembly of Multi-Spheroid Cellular Architectures by Programmable Droplet Merging

© 2020 The Authors. Advanced Materials published by Wiley-VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 33(2021), 4 vom: 08. Jan., Seite e2006434
1. Verfasser: Cui, Haijun (VerfasserIn)
Weitere Verfasser: Wang, Xianxian, Wesslowski, Janine, Tronser, Tina, Rosenbauer, Jakob, Schug, Alexander, Davidson, Gary, Popova, Anna A, Levkin, Pavel A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article droplet merging droplet microarrays high-throughput methods multicellular architectures spheroids
Beschreibung
Zusammenfassung:© 2020 The Authors. Advanced Materials published by Wiley-VCH GmbH.
Artificial multicellular systems are gaining importance in the field of tissue engineering and regenerative medicine. Reconstruction of complex tissue architectures in vitro is nevertheless challenging, and methods permitting controllable and high-throughput fabrication of complex multicellular architectures are needed. Here, a facile and high-throughput method is developed based on a tunable droplet-fusion technique, allowing programmed assembly of multiple cell spheroids into complex multicellular architectures. The droplet-fusion technique allows for construction of various multicellular architectures (double-spheroids, multi-spheroids, hetero-spheroids) in a miniaturized high-density array format. As an example of application, the propagation of Wnt signaling is investigated within hetero-spheroids formed from two fused Wnt-releasing and Wnt-reporter cell spheroids. The developed method provides an approach for miniaturized, high-throughput construction of complex 3D multicellular architectures and can be applied for studying various biological processes including cell signaling, cancer invasion, embryogenesis, and neural development
Beschreibung:Date Completed 24.07.2024
Date Revised 13.10.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-4095
DOI:10.1002/adma.202006434