Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering

Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human tissue engineering and...

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Détails bibliographiques
Publié dans:	BioTechniques. - 1993. - 70(2021), 2 vom: 02. Feb., Seite 107-115
Auteur principal:	Hsieh, Dar-Jen (Auteur)
Autres auteurs:	Srinivasan, Periasamy, Yen, Ko-Chung, Yeh, Yi-Chun, Chen, Yun-Ju, Wang, Hung-Chou, Tarng, Yih-Wen
Format:	Article en ligne
Langue:	English
Publié:	2021
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't 3D bioprinting decellularized tissue scaffolds organ regeneration organ scaffolds porcine organs porcine tissues regenerative medicine supercritical carbon dioxide plus... tissue engineering tissue regeneration Biocompatible Materials


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520			\|a Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human tissue engineering and regenerative medicine applications. Traditional methods for decellularization often disrupt the 3D architecture and damage the structural integrity of the ECM scaffold. To efficiently obtain natural ECM scaffolds from animal tissues and organs with intact architecture, we have developed a platform decellularization process using supercritical CO2 and tested its potential application in tissue engineering. A combination of human mesenchymal stem cells with a decellularized dermal matrix scaffold allowed complete regeneration of skin structure in a porcine full-thickness wound model
650		4	\|a Journal Article
650		4	\|a Research Support, Non-U.S. Gov't
650		4	\|a 3D bioprinting
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650		4	\|a organ scaffolds
650		4	\|a porcine organs
650		4	\|a porcine tissues
650		4	\|a regenerative medicine
650		4	\|a supercritical carbon dioxide
650		4	\|a tissue engineering
650		4	\|a tissue regeneration
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700	1		\|a Srinivasan, Periasamy \|e verfasserin \|4 aut
700	1		\|a Yen, Ko-Chung \|e verfasserin \|4 aut
700	1		\|a Yeh, Yi-Chun \|e verfasserin \|4 aut
700	1		\|a Chen, Yun-Ju \|e verfasserin \|4 aut
700	1		\|a Wang, Hung-Chou \|e verfasserin \|4 aut
700	1		\|a Tarng, Yih-Wen \|e verfasserin \|4 aut
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