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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2020.11.035
|2 doi
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|a pubmed24n1061.xml
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|a eng
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|a Li, Qiang
|e verfasserin
|4 aut
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|a Comparative transcriptome analysis reveals candidate genes involved in anthocyanin biosynthesis in sweetpotato (Ipomoea batatas L.)
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 05.02.2021
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|a Date Revised 05.02.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2020 Elsevier Masson SAS. All rights reserved.
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|a Sweetpotato [Ipomoea batatas (L.) Lam] is an economically important crop for fresh and processed consumption and is widely cultivated worldwide, especially in China. Various sweetpotato cultivars with different storage root colors are presently available. The purple-fleshed sweetpotato obtains its color from anthocyanin accumulation in the storage roots, which is beneficial for both plant and human health. To date, the molecular mechanism of this anthocyanin accumulation has not been studied in detail. In our study, three cDNA libraries generated from 'Xuzi8' with dark-purple flesh, 'Xuzi6' with light-purple flesh, and 'Xu28' with white flesh were sequenced utilizing an Illumina HiSeq™ 2500 platform. Corresponding totals of 28,093,466, 29,239,729 and 27,217,440 raw reads were obtained from the three libraries and assembled into 137,625 unigenes with an average length of 481 bp. Moreover, 79,203 unigenes (57.55%) were found to be annotated in several public databases, and 1285 unigenes were differentially expressed among the Xu28 vs Xuzi8, Xu28 vs Xuzi6, and Xuzi6 vs Xuzi8 libraries. After functional category enrichment analysis of differential expression genes (DEGs), 25 genes were selected as the candidate genes related to anthocyanin accumulation. Furthermore, the expression patterns of some selected DEGs were verified by quantitative real-time PCR (qRT-PCR), and the correlation between expression levels of relevant genes involved in anthocyanin biosynthesis and anthocyanin content was determined. Taken together, the results compose a transcriptomic analysis to investigate the differences in purple flesh formation in the storage roots among different sweetpotato varieties, with the notable outcome that several key genes can now be closely linked to anthocyanin biosynthesis
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|a Journal Article
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|a Anthocyanin
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|a Differential expression genes
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|a Purple-fleshed sweetpotato
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|a Transcriptome
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|a Anthocyanins
|2 NLM
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|a Kou, Meng
|e verfasserin
|4 aut
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|a Li, Chen
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Yun-Gang
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 158(2021) vom: 05. Jan., Seite 508-517
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|u http://dx.doi.org/10.1016/j.plaphy.2020.11.035
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