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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eraa570
|2 doi
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|a pubmed24n1060.xml
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|a (DE-627)NLM318252783
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|a (NLM)33258949
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Song, Yuepeng
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Miniature inverted repeat transposable elements cis-regulate circular RNA expression and promote ethylene biosynthesis, reducing heat tolerance in Populus tomentosa
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 20.05.2021
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|a Date Revised 20.05.2021
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Transposable elements (TEs) and their reverse complementary sequence pairs (RCPs) are enriched around loci that produce circular RNAs (circRNAs) in plants. However, the function of these TE-RCP pairs in modulating circRNA expression remains elusive. Here, we identified 4609 circRNAs in poplar (Populus tomentosa) and showed that miniature inverted repeat transposable elements (MITEs)-RCPs were enriched in circRNA flanking regions. Moreover, we used expression quantitative trait nucleotide (eQTN) mapping to decipher the cis-regulatory role of MITEs. eQTN results showed that 14 single-nucleotide polymorphisms (SNPs) were significantly associated with Circ_0000408 and Circ_0003418 levels and the lead associated SNPs were located in MITE-RCP regions, indicating that MITE-RCP sequence variations affect exon circularization. Overexpression and knockdown analysis showed that Circ_0003418 positively modulated its parental gene, which encodes the RING-type E3 ligase XBAT32, and specifically increased the expression of the PtoXBAT32.5 transcript variant, which lacks the E3 ubiquitin ligase domain. Under heat stress, PtoXBAT32.5 expression was induced with up-regulation of Circ_0003418, resulting in increased production of ethylene and peroxidation of membrane lipids. Our findings thus reveal the cis-regulatory mechanism by which a MITE-RCP pair affects circRNA abundance in poplar and indicate that Circ_0003418 is a negative regulator of poplar heat tolerance via the ubiquitin-mediated protein modification pathway
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a Populus
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| 650 |
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4 |
|a cis-regulatory mechanism
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| 650 |
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4 |
|a CircRNA
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| 650 |
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4 |
|a MITEs
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| 650 |
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4 |
|a exon circularization
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| 650 |
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4 |
|a heat tolerance
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| 650 |
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|a DNA Transposable Elements
|2 NLM
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| 650 |
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|a Ethylenes
|2 NLM
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| 650 |
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7 |
|a RNA, Circular
|2 NLM
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| 700 |
1 |
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|a Bu, Chenhao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Panfei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Peng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Deqiang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 72(2021), 5 vom: 27. Feb., Seite 1978-1994
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:72
|g year:2021
|g number:5
|g day:27
|g month:02
|g pages:1978-1994
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| 856 |
4 |
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|u http://dx.doi.org/10.1093/jxb/eraa570
|3 Volltext
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|a AR
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|d 72
|j 2021
|e 5
|b 27
|c 02
|h 1978-1994
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