Plasma-activated water An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection

Plasma-activated water : An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection

© 2020 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Chemical engineering journal (Lausanne, Switzerland : 1996). - 1999. - 421(2021) vom: 01. Okt., Seite 127742
1. Verfasser: Guo, Li (VerfasserIn)
Weitere Verfasser: Yao, Zhiqian, Yang, Lu, Zhang, Hao, Qi, Yu, Gou, Lu, Xi, Wang, Liu, Dingxin, Zhang, Lei, Cheng, Yilong, Wang, Xiaohua, Rong, Mingzhe, Chen, Hailan, Kong, Michael G
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Chemical engineering journal (Lausanne, Switzerland : 1996)
Schlagworte:Journal Article Plasma-activated water Pseudoviruses Reactive species Receptor-binding domain (RBD) SARS-CoV-2
Beschreibung
Zusammenfassung:© 2020 Elsevier B.V. All rights reserved.
SARS-CoV-2 is a highly contagious virus and is causing a global pandemic. SARS-CoV-2 infection depends on the recognition of and binding to the cellular receptor human angiotensin-converting enzyme 2 (hACE2) through the receptor-binding domain (RBD) of the spike protein, and disruption of this process can effectively inhibit SARS-CoV-2 invasion. Plasma-activated water efficiently inactivates bacteria and bacteriophages by causing damage to biological macromolecules, but its effect on coronavirus has not been reported. In this study, pseudoviruses with the SARS-CoV-2 S protein were used as a model, and plasma-activated water (PAW) effectively inhibited pseudovirus infection through S protein inactivation. The RBD was used to study the molecular details, and the RBD binding activity was inactivated by plasma-activated water through the RBD modification. The short-lived reactive species in the PAW, such as ONOO-, played crucial roles in this inactivation. Plasma-activated water after room-temperature storage of 30 days remained capable of significantly reducing the RBD binding with hACE2. Together, our findings provide evidence of a potent disinfection strategy to combat the epidemic caused by SARS-CoV-2
Beschreibung:Date Revised 15.06.2021
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:1385-8947
DOI:10.1016/j.cej.2020.127742