Increased sensitivity using real-time dPCR for detection of SARS-CoV-2

A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end...

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Publié dans:BioTechniques. - 1993. - 70(2021), 1 vom: 21. Jan., Seite 7-20
Auteur principal: Duong, Kyra (Auteur)
Autres auteurs: Ou, Jiajia, Li, Zhaoliang, Lv, Zhaoqing, Dong, Hao, Hu, Tao, Zhang, Yunyun, Hanna, Ava, Gordon, Skyler, Crynen, Gogce, Head, Steven R, Ordoukhanian, Phillip, Wang, Yan
Format: Article en ligne
Langue:English
Publié: 2021
Accès à la collection:BioTechniques
Sujets:Comparative Study Journal Article SARS-CoV-2 dPCR gene expression infectious disease diagnostics qPCR real-time PCR real-time digital PCR