Increased sensitivity using real-time dPCR for detection of SARS-CoV-2
A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 70(2021), 1 vom: 22. Jan., Seite 7-20
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| 1. Verfasser: |
Duong, Kyra
(VerfasserIn) |
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| Weitere Verfasser: |
Ou, Jiajia,
Li, Zhaoliang,
Lv, Zhaoqing,
Dong, Hao,
Hu, Tao,
Zhang, Yunyun,
Hanna, Ava,
Gordon, Skyler,
Crynen, Gogce,
Head, Steven R,
Ordoukhanian, Phillip,
Wang, Yan |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Comparative Study
Journal Article
SARS-CoV-2
dPCR
gene expression
infectious disease diagnostics
qPCR
real-time PCR
real-time digital PCR |
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