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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eraa548
|2 doi
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|a pubmed24n1059.xml
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|a (DE-627)NLM317839845
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|a (NLM)33216934
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Karafiátová, Miroslava
|e verfasserin
|4 aut
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|a The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.05.2021
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|a Date Revised 20.05.2021
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|a published: Print
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|a Dryad: 10.5061/dryad.rxwdbrv5j
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|a Citation Status MEDLINE
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|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a More than a century has passed since the B chromosomes were first discovered. Today we know much of their variability, morphology, and transmission to plant progeny. With the advent of modern technologies, B chromosome research has accelerated, and some of their persistent mysteries have since been uncovered. Building on this momentum, here we extend current knowledge of B chromosomes in Sorghum purpureosericeum to the sequence level. To do this, we estimated the B chromosome size at 421 Mb, sequenced DNA from flow-sorted haploid pollen nuclei of both B-positive (B+) and B-negative (B0) plants, and performed a repeat analysis on the Illumina raw sequence data. This analysis revealed nine putative B-specific clusters, which were then used to develop B chromosome-specific markers. Additionally, cluster SpuCL4 was identified and verified to be a centromeric repeat. We also uncovered two repetitive clusters (SpuCL168 and SpuCL115), which hybridized exclusively on the B chromosome under fluorescence in situ hybridization and can be considered as robust cytogenetic markers. Given that B chromosomes in Sorghum are rather unstable across all tissues, our findings could facilitate expedient identification of B+ plants and enable a wide range of studies to track this chromosome type in situ
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Sorghum purpureosericeum
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|a B chromosomes
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|a cytogenetics
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| 650 |
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|a flow cytometry
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|a pollen nuclei
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| 650 |
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|a repeat analysis
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| 650 |
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|a Genetic Markers
|2 NLM
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1 |
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|a Bednářová, Martina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Said, Mahmoud
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Čížková, Jana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Holušová, Kateřina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Blavet, Nicolas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bartoš, Jan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 72(2021), 5 vom: 27. Feb., Seite 1606-1616
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:72
|g year:2021
|g number:5
|g day:27
|g month:02
|g pages:1606-1616
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|u http://dx.doi.org/10.1093/jxb/eraa548
|3 Volltext
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|d 72
|j 2021
|e 5
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|h 1606-1616
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