Amyloid Formation by Short Peptides in the Presence of Dipalmitoylphosphatidylcholine Membranes

The aggregation of two short peptides, [RF] and [RF]4 (where R = arginine and F = phenylalanine), at dipalmitoylphosphatidylcholine (DPPC) model membranes was investigated at the air-water interface using the Langmuir technique and vesicles in aqueous solutions. The molar ratio of the peptide and li...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1999. - 36(2020), 48 vom: 08. Dez., Seite 14793-14801
1. Verfasser: Gerbelli, Barbara B (VerfasserIn)
Weitere Verfasser: Oliveira, Cristiano L P, Silva, Emerson R, Hamley, Ian W, Alves, Wendel A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Amyloid Lipid Bilayers Peptides 1,2-Dipalmitoylphosphatidylcholine 2644-64-6
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520 |a The aggregation of two short peptides, [RF] and [RF]4 (where R = arginine and F = phenylalanine), at dipalmitoylphosphatidylcholine (DPPC) model membranes was investigated at the air-water interface using the Langmuir technique and vesicles in aqueous solutions. The molar ratio of the peptide and lipid components was varied to provide insights into the peptide-membrane interactions, which might be related to their cytotoxicity. Both peptides exhibited affinity to the DPPC membrane interface and rapidly adopted β-sheet-rich structures upon adsorption onto the surface of the zwitterionic membrane. Results from adsorption isotherm and small-angle X-ray scattering experiments showed changes in the structural and thermodynamic parameters of the membrane with increasing peptide concentration. Using atomic force microscopy, we showed the appearance of pores through the bilayer membranes and peptide aggregation at different interfaces, suggesting that the hydrophobic residues might have an effect on both pore size and layer structure, facilitating the membrane disruption and leading to different cytotoxicity effects 
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700 1 |a Oliveira, Cristiano L P  |e verfasserin  |4 aut 
700 1 |a Silva, Emerson R  |e verfasserin  |4 aut 
700 1 |a Hamley, Ian W  |e verfasserin  |4 aut 
700 1 |a Alves, Wendel A  |e verfasserin  |4 aut 
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