Leaf width gene LW5/D1 affects plant architecture and yield in rice by regulating nitrogen utilization efficiency

Leaf width gene LW5/D1 affects plant architecture and yield in rice by regulating nitrogen utilization efficiency

Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 157(2020) vom: 01. Dez., Seite 359-369
1. Verfasser: Zhu, Yuchen (VerfasserIn)
Weitere Verfasser: Li, Ting, Xu, Jing, Wang, Jiajia, Wang, Li, Zou, Weiwei, Zeng, Dali, Zhu, Li, Chen, Guang, Hu, Jiang, Gao, Zhenyu, Dong, Guojun, Ren, Deyong, Shen, Lan, Zhang, Qiang, Guo, Longbiao, Hu, Songping, Qian, Qian, Zhang, Guangheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Grain development Leaf morphology Map-based cloning Nitrogen transport efficiency Rice (Oryza sativa L.) Plant Proteins Chlorophyll 1406-65-1 Nitrogen N762921K75
Beschreibung
Zusammenfassung:Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.
Leaves are the primary structures responsible for photosynthesis, making leaf morphology one of the most important traits of rice plant architecture. Both plant architecture and nutrient utilization jointly affect rice yield, however, their molecular association is still poorly understood. We identified a rice mutant, leaf width 5 (lw5), that displayed small grains and wide leaves and possesses characteristics typical of a small "sink" and a large "source". Map-based cloning and CRISPR-Cas9 gene editing indicated that LW5 affects both the plant architecture and yield. It is an allele of D1, encoding the rice G protein α subunit. The loss of LW5 functioning leads to an increase in the rate of photosynthesis, vascular bundles, and chlorophyll content. However, the grain-straw ratio and the rate of grain filling decreased significantly. The detection results of 15N-ammonium nitrate and an expression analysis of genes associated with nitrogen demonstrated that LW5 serves an important role in nitrate uptake and transport. LW5 affects plant architecture and grain size by regulating nitrogen transfer. These results provide a theoretical foundation for further research surrounding the molecular mechanism of "source-sink" balance in rice and suggest novel methods of molecular design for the cultivation of breeding super rice in ideal plant types
Beschreibung:Date Completed 27.01.2021
Date Revised 27.01.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2020.10.035