A role for calcium-dependent protein kinases in differential CO2 - and ABA-controlled stomatal closing and low CO2 -induced stomatal opening in Arabidopsis

A role for calcium-dependent protein kinases in differential CO2 - and ABA-controlled stomatal closing and low CO2 -induced stomatal opening in Arabidopsis

© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 229(2021), 5 vom: 13. März, Seite 2765-2779
1. Verfasser: Schulze, Sebastian (VerfasserIn)
Weitere Verfasser: Dubeaux, Guillaume, Ceciliato, Paulo H O, Munemasa, Shintaro, Nuhkat, Maris, Yarmolinsky, Dmitry, Aguilar, Jaimee, Diaz, Renee, Azoulay-Shemer, Tamar, Steinhorst, Leonie, Offenborn, Jan Niklas, Kudla, Jörg, Kollist, Hannes, Schroeder, Julian I
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis abscisic acid (ABA) calcineurin B-like proteins (CBLs) calcium calcium-dependent protein kinases (CPKs) carbon dioxide (CO2) mehr... gas exchange guard cells Arabidopsis Proteins Carbon Dioxide 142M471B3J Abscisic Acid 72S9A8J5GW Protein Kinases EC 2.7.- calcium-dependent protein kinase EC 2.7.1.-
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100 1 |a Schulze, Sebastian  |e verfasserin  |4 aut 
245 1 2 |a A role for calcium-dependent protein kinases in differential CO2 - and ABA-controlled stomatal closing and low CO2 -induced stomatal opening in Arabidopsis 
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500 |a Citation Status MEDLINE 
520 |a © 2020 The Authors New Phytologist © 2020 New Phytologist Foundation. 
520 |a Low concentrations of CO2 cause stomatal opening, whereas [CO2 ] elevation leads to stomatal closure. Classical studies have suggested a role for Ca2+ and protein phosphorylation in CO2 -induced stomatal closing. Calcium-dependent protein kinases (CPKs) and calcineurin-B-like proteins (CBLs) can sense and translate cytosolic elevation of the second messenger Ca2+ into specific phosphorylation events. However, Ca2+ -binding proteins that function in the stomatal CO2 response remain unknown. Time-resolved stomatal conductance measurements using intact plants, and guard cell patch-clamp experiments were performed. We isolated cpk quintuple mutants and analyzed stomatal movements in response to CO2 , light and abscisic acid (ABA). Interestingly, we found that cpk3/5/6/11/23 quintuple mutant plants, but not other analyzed cpk quadruple/quintuple mutants, were defective in high CO2 -induced stomatal closure and, unexpectedly, also in low CO2 -induced stomatal opening. Furthermore, K+ -uptake-channel activities were reduced in cpk3/5/6/11/23 quintuple mutants, in correlation with the stomatal opening phenotype. However, light-mediated stomatal opening remained unaffected, and ABA responses showed slowing in some experiments. By contrast, CO2 -regulated stomatal movement kinetics were not clearly affected in plasma membrane-targeted cbl1/4/5/8/9 quintuple mutant plants. Our findings describe combinatorial cpk mutants that function in CO2 control of stomatal movements and support the results of classical studies showing a role for Ca2+ in this response 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Arabidopsis 
650 4 |a abscisic acid (ABA) 
650 4 |a calcineurin B-like proteins (CBLs) 
650 4 |a calcium 
650 4 |a calcium-dependent protein kinases (CPKs) 
650 4 |a carbon dioxide (CO2) 
650 4 |a gas exchange 
650 4 |a guard cells 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Carbon Dioxide  |2 NLM 
650 7 |a 142M471B3J  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a Protein Kinases  |2 NLM 
650 7 |a EC 2.7.-  |2 NLM 
650 7 |a calcium-dependent protein kinase  |2 NLM 
650 7 |a EC 2.7.1.-  |2 NLM 
700 1 |a Dubeaux, Guillaume  |e verfasserin  |4 aut 
700 1 |a Ceciliato, Paulo H O  |e verfasserin  |4 aut 
700 1 |a Munemasa, Shintaro  |e verfasserin  |4 aut 
700 1 |a Nuhkat, Maris  |e verfasserin  |4 aut 
700 1 |a Yarmolinsky, Dmitry  |e verfasserin  |4 aut 
700 1 |a Aguilar, Jaimee  |e verfasserin  |4 aut 
700 1 |a Diaz, Renee  |e verfasserin  |4 aut 
700 1 |a Azoulay-Shemer, Tamar  |e verfasserin  |4 aut 
700 1 |a Steinhorst, Leonie  |e verfasserin  |4 aut 
700 1 |a Offenborn, Jan Niklas  |e verfasserin  |4 aut 
700 1 |a Kudla, Jörg  |e verfasserin  |4 aut 
700 1 |a Kollist, Hannes  |e verfasserin  |4 aut 
700 1 |a Schroeder, Julian I  |e verfasserin  |4 aut 
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