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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.0c01953
|2 doi
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|a pubmed25n1058.xml
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|a (DE-627)NLM317539671
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|a (NLM)33186493
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Ogharandukun, Eric
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Establishing Rules for Self-Adhesion and Aggregation of N-Glycan Sugars Using Virus Glycan Shields
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 21.06.2021
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|a Date Revised 05.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The surfaces of cells and pathogens are covered with short polymers of sugars known as glycans. Complex N-glycans have a core of three mannose sugars with distal repeats of N-acetylglucosamine and galactose sugars terminating with sialic acid (SA). Long-range tough and short-range brittle self-adhesions were observed between SA and mannose residues, respectively, in ill-defined artificial monolayers. We investigated if and how these adhesions translate when the residues are presented in N-glycan architecture with SA at the surface and mannose at the core and with other glycan sugars. Two pseudotyped viruses with complex N-glycan shields were brought together in force spectroscopy (FS). At higher ramp rates, slime-like adhesions were observed between the shields, whereas Velcro-like adhesions were observed at lower rates. The higher approach rates compress the virus as a whole, and the self-adhesion between the surface SA is sampled. At the lower ramp rates, however, the complex glycan shield is penetrated and adhesion from the mannose core is accessed. The slime-like and Velcro-like adhesions were lost when SA and mannose were cleaved, respectively. While virus self-adhesion in forced contact was modulated by glycan penetrability, the self-aggregation of the freely diffusing virus was only determined by the surface sugar. Mannose-terminal viruses self-aggregated in solution, and SA-terminal ones required Ca2+ ions to self-aggregate. Viruses with galactose or N-acetylglucosamine surfaces did not self-aggregate, irrespective of whether or not a mannose core was present below the N-acetylglucosamine surface. Well-defined rules appear to govern the self-adhesion and -aggregation of N-glycosylated surfaces, regardless of whether the sugars are presented in an ill-defined monolayer, or N-glycan, or even polymer architecture
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Polysaccharides
|2 NLM
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|a Sugars
|2 NLM
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|a N-Acetylneuraminic Acid
|2 NLM
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| 650 |
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|a GZP2782OP0
|2 NLM
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| 650 |
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|a Mannose
|2 NLM
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| 650 |
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|a PHA4727WTP
|2 NLM
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| 700 |
1 |
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|a Tewolde, Wintana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Damtae, Elbethel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Songping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ivanov, Andrey
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kumari, Namita
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nekhai, Sergei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chandran, Preethi L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 36(2020), 46 vom: 24. Nov., Seite 13769-13783
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
1 |
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|g volume:36
|g year:2020
|g number:46
|g day:24
|g month:11
|g pages:13769-13783
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|u http://dx.doi.org/10.1021/acs.langmuir.0c01953
|3 Volltext
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|a AR
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|d 36
|j 2020
|e 46
|b 24
|c 11
|h 13769-13783
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