|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM317482335 |
| 003 |
DE-627 |
| 005 |
20250228085422.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plantsci.2020.110632
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1058.xml
|
| 035 |
|
|
|a (DE-627)NLM317482335
|
| 035 |
|
|
|a (NLM)33180711
|
| 035 |
|
|
|a (PII)S0168-9452(20)30238-7
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Jiang, Xiaolan
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Functional characterization of three flavonol synthase genes from Camellia sinensis
|b Roles in flavonol accumulation
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 01.04.2021
|
| 500 |
|
|
|a Date Revised 01.04.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2020 Elsevier B.V. All rights reserved.
|
| 520 |
|
|
|a Flavonol derivatives are a group of flavonoids benefiting human health. Their abundant presence in tea is associated with astringent taste. To date, mechanism pertaining to the biosynthesis of flavonols in tea plants remains unknown. In this study, we used bioinformatic analysis mining the tea genome and obtained three cDNAs that were annotated to encode flavonol synthases (FLS). Three cDNAs, namely CsFLSa, b, and c, were heterogenously expressed in E. coli to induce recombinant proteins, which were further used to incubate with three substrates, dihydrokampferol (DHK), dihydroquercetin (DHQ), and dihydromyricetin (DHM). The resulting data showed that three rCsFLSs preferred to catalyze (DHK). Overexpression of each cDNA in tobacco led to the increase of kampferol and the reduction of anthocyanins in flowers. Further metabolic profiling of flavan-3-ols in young tea shoots characterized that kaempferol derivatives were the most abundant, followed by quercetin and then myricetin derivatives. Taken together, these data characterized the key step committed to the biosynthesis of flavonols in tea leaves. Moreover, these data enhance understanding the metabolic accumulation relevance between flavonols and other main flavonoids such as flavan-3-ols in tea leaves
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Accumulation pattern
|
| 650 |
|
4 |
|a Anthocyanins
|
| 650 |
|
4 |
|a Camellia sinensis
|
| 650 |
|
4 |
|a Flavan-3-ols
|
| 650 |
|
4 |
|a Flavonol synthase
|
| 650 |
|
4 |
|a Flavonols
|
| 650 |
|
7 |
|a Flavonols
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Tea
|2 NLM
|
| 650 |
|
7 |
|a Oxidoreductases
|2 NLM
|
| 650 |
|
7 |
|a EC 1.-
|2 NLM
|
| 700 |
1 |
|
|a Shi, Yufeng
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fu, Zhouping
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Wei-Wei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lai, Sanyan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wu, Yahui
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Yunsheng
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liu, Yajun
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gao, Liping
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xia, Tao
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 300(2020) vom: 12. Nov., Seite 110632
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
|
| 773 |
1 |
8 |
|g volume:300
|g year:2020
|g day:12
|g month:11
|g pages:110632
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plantsci.2020.110632
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 300
|j 2020
|b 12
|c 11
|h 110632
|