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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2020.110641
|2 doi
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|a pubmed24n1058.xml
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|a (NLM)33180701
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|a (PII)S0168-9452(20)30247-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Guo, Wei
|e verfasserin
|4 aut
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|a The hawthorn CpLRR-RLK1 gene targeted by ACLSV-derived vsiRNA positively regulate resistance to bacteria disease
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.04.2021
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|a Date Revised 01.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2020 Elsevier B.V. All rights reserved.
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|a Virus-derived small interfering RNAs (vsiRNAs) can target not only viruses but also plant genes. Apple chlorotic leaf spot virus (ACLSV) is an RNA virus that infects Rosaceae plants extensively, including apple, pear and hawthorn. Here, we report an ACLSV-derived vsiRNA [vsiR1360(-)] that targets and down-regulates the leucine-rich repeat receptor-like kinase 1 (LRR-RLK1) gene of hawthorn (Crataegus pinnatifida). The targeting and cleavage of the CpLRR-RLK1 gene by vsiR1360(-) were validated by RNA ligase-mediated 5' rapid amplification of cDNA ends and tobacco transient transformation assays. And the CpLRR-RLK1 protein fused to green fluorescent protein localized to the cell membrane. Conserved domain and phylogenetic tree analyses showed that CpLRR-RLK1 is closely related to the proteins of the LRRII-RLK subfamily. The biological function of CpLRR-RLK1 was explored by heterologous overexpression of CpLRR-RLK1 gene in Arabidopsis. The results of inoculation of Pst DC3000 in Arabidopsis leaves showed that the symptoms of CpLRR-RLK1 overexpression plants infected with Pst DC3000 were significantly reduced compared with the wild type. In addition, the detection of reactive oxygen species and callose deposition and the expression analysis of defense-related genes showed that the CpLRR-RLK1 gene can indeed enhance the resistance of Arabidopsis to bacteria disease
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|a Journal Article
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|a ACLSV
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|a Crataegus pinnatifida
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|a LRR-RLK
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|a bacteria disease
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|a target gene
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|a vsiRNA
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|a Chen, Wenjun
|e verfasserin
|4 aut
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|a Zhang, Zhihong
|e verfasserin
|4 aut
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|a Guo, Nan
|e verfasserin
|4 aut
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|a Liu, Lifu
|e verfasserin
|4 aut
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|a Ma, Yue
|e verfasserin
|4 aut
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|a Dai, Hongyan
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 300(2020) vom: 12. Nov., Seite 110641
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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|g volume:300
|g year:2020
|g day:12
|g month:11
|g pages:110641
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|u http://dx.doi.org/10.1016/j.plantsci.2020.110641
|3 Volltext
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