Case study development of a challenge test against Edwardsiella ictaluri in Mekong striped catfish (Pangasianodon hypophthalmus), for use in breeding : Estimates of the genetic correlation between susceptibilities in replicated tanks
© 2020 John Wiley & Sons Ltd.
| Publié dans: | Journal of fish diseases. - 1998. - 44(2021), 5 vom: 01. Mai, Seite 553-561 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2021
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| Accès à la collection: | Journal of fish diseases |
| Sujets: | Journal Article bacillary necrosis test environment |
| Résumé: | © 2020 John Wiley & Sons Ltd. Bacillary necrosis is a problematic disease in farming of Mekong striped catfish (Pangasianodon hypophthalmus). The pathogenic bacterium is Edwardsiella ictaluri, causing numerous white spots in swelled liver, kidney and spleen. An alternative to antibiotic treatment and vaccine is to select for improved genetic resistance to the disease that requires to establish a proper challenge test. Here, four challenge tests of Mekong striped catfish against E. ictaluri are reported proposing 3 days of acclimatization of test fish prior to the challenge, with restricted water level in the test, keeping a temperature of 26°C. In the challenge, cohabitant shedders should be released directly into the test tank and make up around ⅓ of the fish, and bacteria should be added directly to water. The last two experiments, with the highest mortality, suggest that any factor involving the dead cohabitants should be removed and that additional experimentation should focus on bacteria (density) and timing for addition of bacteria to water. Genetic analyses revealed that resistance to bacillary necrosis tested in replicated tanks in the same experiment can be considered the same genetic trait |
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| Description: | Date Completed 26.05.2021 Date Revised 26.05.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/jfd.13292 |