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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13291
|2 doi
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|a pubmed24n1057.xml
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|a (NLM)33152130
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|a eng
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| 100 |
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|a Qu, Yang
|e verfasserin
|4 aut
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| 245 |
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|a Development of a real-time recombinase polymerase amplification assay for rapid detection of Aeromonas hydrophila
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 19.05.2021
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|a Date Revised 19.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 John Wiley & Sons Ltd.
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|a Aeromonas hydrophila is ubiquitous in the aquaculture industry and a constant cause of severe disease and economic losses. The early diagnosis of these infections is crucial for disease surveillance and prevention. We developed a real-time recombinase polymerase amplification (real-time RPA) assay for detection of A. hydrophila using the haemolysin gene. The assay was performed at 37°C for 20 min and was highly specific with no cross-reaction with other fish pathogens or with other Aeromonas species. The assay detection limit was 102 copies of the Aeromonas hydrophila per reaction. Compared with traditional culture-based method or real-time PCR, the diagnostic sensitivity and specificity of the real-time RPA were 73.7 and 100%, as well as 64.7 and 93%. Our newly developed real-time RPA was specific and sensitive and can be used in large-scale and point-of-care field investigations of A. hydrophila infections to enable earlier diagnoses
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|a Journal Article
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|a Aeromonas hydrophila
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|a detection
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|a haemolysin gene
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| 650 |
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4 |
|a real-time recombinase polymerase amplification
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| 700 |
1 |
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|a Wang, Qing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Yingying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yingying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yin, Jiyuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ren, Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Chun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Xiaofang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yahui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zeng, Weiwei
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 44(2021), 4 vom: 01. Apr., Seite 469-477
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:44
|g year:2021
|g number:4
|g day:01
|g month:04
|g pages:469-477
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|u http://dx.doi.org/10.1111/jfd.13291
|3 Volltext
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