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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.0c02201
|2 doi
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|a pubmed24n1054.xml
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|a (DE-627)NLM316400394
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|a (NLM)33070609
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zhong, Wen
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Biosurfaces Fabricated by Polymerization-Induced Surface Self-Assembly
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| 264 |
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1 |
|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 04.11.2020
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|a Date Revised 04.11.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Surface biofunctionalization provides an approach to the fabrication of surfaces with improved biological and clinical performances. Biosurfaces have found increasing applications in many areas such as sensing, cell growth, and disease detection. Efficient synthesis of biosurfaces without damages to the structures and functionalities of biomolecules is a great challenge. Polymerization-induced surface self-assembly (PISSA) provides an effective approach to the synthesis of surface nanostructures with different compositions, morphologies, and properties. In this research, application of PISSA in the fabrication of biosurfaces is investigated. Two different reversible addition-fragmentation chain transfer (RAFT) agents, RAFT chain transfer agent (CTA) on silica particles (SiO2-CTA) and CTA on bovine serum albumin (BSA-CTA), were employed in RAFT dispersion polymerization of N-isopropylacrylamide (NIPAM) in water at a temperature above the lower critical solution temperature (LCST) of poly-(isopropylacrylamide) (PNIPAM). After polymerization, PNIPAM layers with BSA on the top surfaces are fabricated on the surfaces of silica particles. Transmission electron microscopy results show that the average PNIPAM layer thickness increases with monomer conversion. Kinetics study indicates that there is a turn point on a plot of ln([M]0/[M]t) versus polymerization time. After the critical point, surface coassembly of PNIPAM brushes and BSA-PNIPAM bioconjugates is performed on the silica particles. The secondary structure and the activity of BSA immobilized on top of the PNIPAM layers are basically kept unchanged in the PISSA process. To prepare permanently immobilized protein surfaces, PNIPAM layers on silica particles are cross-linked. BSA on the top surfaces presents a reversible "on-off" switching property. At a temperature below the LCST of PNIPAM, the activity of the immobilized BSA is retained; however, the BSA activity decreases significantly at a temperature above the LCST because of the hydrophobic interaction between PNIPAM and BSA. Based on this approach, many different biosurfaces can be fabricated and the materials will find applications in many fields, such as enzyme immobilization, drug delivery, and tissue engineering
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Hou, Wangmeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Yingze
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Hanying
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 36(2020), 42 vom: 27. Okt., Seite 12649-12657
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:36
|g year:2020
|g number:42
|g day:27
|g month:10
|g pages:12649-12657
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| 856 |
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|u http://dx.doi.org/10.1021/acs.langmuir.0c02201
|3 Volltext
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