|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM316182796 |
| 003 |
DE-627 |
| 005 |
20240229142915.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1094/PDIS-08-20-1717-PDN
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1303.xml
|
| 035 |
|
|
|a (DE-627)NLM316182796
|
| 035 |
|
|
|a (NLM)33048597
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Xu, Dandan
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a First report of powdery mildew of crape jasmine (Tabernaemontana divaricata) caused by Erysiphe elevata in China
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 22.02.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status Publisher
|
| 520 |
|
|
|a Crape jasmine (Tabernaemontana divaricata) is a popular flowering shrub widely grown in southern China. Its leaves and roots are used in Chinese traditional medicine. In December, 2019, powdery mildew symptoms were observed on five crape jasmine shrubs on the campus of Shenzhen Polytechnic (22°35'N; 113°56'E), in Guangdong province. Approximately 45% of leaves were infected. Symptoms initially appeared as circular to irregular white patches on the leaf petiole, and subsequently coalesced to develop into abundant hyphal growth on both sides of the leaves, which soon wilted. Hyphae were septate, branched, with simple kidney-shaped to moderately lobed appressoria. Conidia formed singly, ellipsoid-ovoid to subcylindrical, 27-37 × 14-20 μm (mean 32±2.5 × 17±1.6 μm), with a length/width ratio varying from 1.3 to 2.4. Conidiophores were erect, unbranched, consisted of two cells, 60 to 84 μm long (mean 73±4 μm), and with straight to severely kinked cylindrical foot-cells at the base, 29-35 × 3-7 μm (mean 32±3 × 6±2 μm). Chasmothecia were not observed on sampled plants. These morphological characteristics were typical to the conidial stage of the genus Erysiphe (Braun and Cook, 2012). For molecular identification, genomic DNA was extracted from conidia washed from infected leaves and using Fungal DNA Kit (Omega Bio-tek Inc., Guangzhou, China). Semi-nested PCR amplification of the internal transcribed spacer (ITS) region of rDNA was conducted by using primer sets P3 (Kusaba et al., 1995)/ITS5 and ITS5/ITS4 (White et al., 1990) for the first and second reactions, respectively. BLASTn analysis of the obtained 719 bp sequence (GenBank Accession No. MT802112) showed 99.7% identity with those of E. elevata (KY660910, MH985631, MK253282). On the basis of morphological and molecular analyses, the fungus was identified as Erysiphe elevata. To confirm pathogenicity, infected leaves were gently pressed onto healthy leaves of three healthy plants in separate pots, and three noninoculated plants were used as controls. All plants were maintained in a greenhouse at 25 ℃, and relative humidity of 50 to 65%. After 11 days, similar disease symptoms were observed on the inoculated plants while no symptoms developed on control plants. The fungus observed on the inoculated shrubs was identical morphologically to that o the original infected leaves. E. elevata is a common powdery mildew species infecting Catalpa spp. (Cook et al., 2006), Plumeria rubra (Wu et al., 2019; Yeh et al., 2019) and Eucalyptus camaldulensis (Meeboon and Takamatsu, 2017). However, no powdery mildew were found on P. rubra nearby. To our knowledge, this is the first report of this fungus infecting T. divaricata
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Causal Agent
|
| 650 |
|
4 |
|a Crop Type
|
| 650 |
|
4 |
|a Fungi
|
| 650 |
|
4 |
|a Ornamentals
|
| 650 |
|
4 |
|a Pathogen detection
|
| 650 |
|
4 |
|a Subject Areas
|
| 700 |
1 |
|
|a Zeng, Yani
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Jianfan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Xu, Jiehua
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Qiao, Fang
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g (2020) vom: 13. Okt.
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
|
| 773 |
1 |
8 |
|g year:2020
|g day:13
|g month:10
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS-08-20-1717-PDN
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|j 2020
|b 13
|c 10
|