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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/jpy.13083
|2 doi
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|a pubmed24n1053.xml
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|a (DE-627)NLM316132713
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|a (NLM)33043442
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Carrasco Flores, David
|e verfasserin
|4 aut
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|a A marine Chlamydomonas sp. emerging as an algal model
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.04.2021
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|a Date Revised 20.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America.
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|a The freshwater microalga Chlamydomonas reinhardtii, which lives in wet soil, has served for decades as a model for numerous biological processes, and many tools have been introduced for this organism. Here, we have established a stable nuclear transformation for its marine counterpart, Chlamydomonas sp. SAG25.89, by fusing specific cis-acting elements from its Actin gene with the gene providing hygromycin resistance and using an elaborated electroporation protocol. Like C. reinhardtii, Chlamydomonas sp. has a high GC content, allowing reporter genes and selection markers to be applicable in both organisms. Chlamydomonas sp. grows purely photoautotrophically and requires ammonia as a nitrogen source because its nuclear genome lacks some of the genes required for nitrogen metabolism. Interestingly, it can grow well under both low and very high salinities (up to 50 g · L-1 ) rendering it as a model for osmotolerance. We further show that Chlamydomonas sp. grows well from 15 to 28°C, but halts its growth at 32°C. The genome of Chlamydomonas sp. contains some gene homologs the expression of which is regulated according to the ambient temperatures and/or confer thermal acclimation in C. reinhardtii. Thus, knowledge of temperature acclimation can now be compared to the marine species. Furthermore, Chlamydomonas sp. can serve as a model for studying marine microbial interactions and for comparing mechanisms in freshwater and marine environments. Chlamydomonas sp. was previously shown to be immobilized rapidly by a cyclic lipopeptide secreted from the antagonistic bacterium Pseudomonas protegens PF-5, which deflagellates C. reinhardtii
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a electroporation
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| 650 |
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4 |
|a marine Chlamydomonas
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| 650 |
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4 |
|a microalgae
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| 650 |
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4 |
|a microbial interactions
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4 |
|a nitrogen metabolism
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| 650 |
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4 |
|a nuclear transformation
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| 650 |
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4 |
|a salinity
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| 650 |
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4 |
|a temperature
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| 700 |
1 |
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|a Fricke, Markus
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wesp, Valentin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Desirò, Daniel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kniewasser, Anja
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hölzer, Martin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Marz, Manja
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mittag, Maria
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of phycology
|d 1966
|g 57(2021), 1 vom: 12. Feb., Seite 54-69
|w (DE-627)NLM098182994
|x 1529-8817
|7 nnns
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| 773 |
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|g volume:57
|g year:2021
|g number:1
|g day:12
|g month:02
|g pages:54-69
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|u http://dx.doi.org/10.1111/jpy.13083
|3 Volltext
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