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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2020.08.022
|2 doi
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|a pubmed24n1053.xml
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|a (DE-627)NLM316085901
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|a (NLM)33038690
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|a (PII)S0981-9428(20)30404-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Duan, Wenjing
|e verfasserin
|4 aut
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|a Dynamic proteome changes of wheat developing grains in response to water deficit and high-nitrogen fertilizer conditions
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 19.01.2021
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|a Date Revised 19.01.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2020. Published by Elsevier Masson SAS.
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|a This study investigated grain proteomic profiles in response to water deficit, high nitrogen (N) fertilizer, and their combined treatments in elite Chinese bread wheat cultivar Jingdong 17, using a two-dimensional difference gel electrophoresis (2D-DIGE)-based approach. Water deficit negatively affected the main agronomic traits of wheat and grain yield, while high-N fertilizer had the opposite effects. The application of a high-N fertilizer under water deficit conditions moderately improved kernel development and grain yield. 2D-DIGE led to the identification of 124 differentially accumulated protein (DAP) spots during five different grain developmental stages, corresponding to 97 unique proteins. The more significant changes of DAPs occurred at 10-20 days after flowering. DAPs were involved in carbohydrate metabolism, protein turnover, protein folding, cell cycle control, stress response, nitrogen metabolism, photosynthesis, and energy metabolism. In particular, water deficit caused a significant downregulation of proteins involved in starch biosynthesis, whereas high-N fertilizer led to a significant upregulation of proteins involved in nitrogen metabolism, carbohydrate metabolism, and starch biosynthesis. The combined treatment resulted in a moderate upregulation of DAPs related to carbohydrate metabolism, starch biosynthesis, and nitrogen metabolism. Our results indicated that high-N fertilization could alleviate yield loss caused by water deficit by promoting the accumulation of proteins involved in nitrogen and carbohydrate metabolism
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|a Journal Article
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|a 2D-DIGE
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|a Grains
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|a High nitrogen
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|a Proteomic analysis
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|a Water deficit
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|a Wheat
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|a Fertilizers
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Proteome
|2 NLM
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| 650 |
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|a Water
|2 NLM
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| 650 |
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|a 059QF0KO0R
|2 NLM
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| 650 |
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Zhu, Gengrui
|e verfasserin
|4 aut
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| 700 |
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|a Zhu, Dong
|e verfasserin
|4 aut
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| 700 |
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|a Yan, Yueming
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 156(2020) vom: 01. Nov., Seite 471-483
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:156
|g year:2020
|g day:01
|g month:11
|g pages:471-483
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|u http://dx.doi.org/10.1016/j.plaphy.2020.08.022
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