|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM315763051 |
| 003 |
DE-627 |
| 005 |
20231225155622.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2021 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/eraa451
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1052.xml
|
| 035 |
|
|
|a (DE-627)NLM315763051
|
| 035 |
|
|
|a (NLM)33005924
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ma, Haiyan
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Functional divergence of diacylglycerol acyltransferases in the unicellular green alga Haematococcus pluvialis
|
| 264 |
|
1 |
|c 2021
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 13.05.2021
|
| 500 |
|
|
|a Date Revised 13.05.2021
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final committed step in triacylglycerol biosynthesis in eukaryotes. In microalgae, the copy number of DGAT genes is extraordinarily expanded, yet the functions of many DGATs remain largely unknown. This study revealed that microalgal DGAT can function as a lysophosphatidic acyltransferase (LPAAT) both in vitro and in vivo while losing its original function as DGAT. Among the five DGAT-encoding genes identified and cloned from the green microalga Haematococcus pluvialis, four encoded HpDGATs that showed triacylglycerol synthase activities in yeast functional complementation analyses; the exception was one of the type II DGAT encoding genes, HpDGTT2. The hydrophobic recombinant HpDGTT2 protein was purified in soluble form and was found to function as a LPAAT via enzymatic assay. Introducing this gene into the green microalga Chlamydomonas reinhardtii led to retarded cellular growth, enlarged cell size, and enhanced triacylglycerol accumulation, identical to the phenotypes of transgenic strains overexpressing CrLPAAT. This study provides a framework for dissecting uncharacterized DGATs, and could pave the way to decrypting the structure-function relationship of this large group of enzymes that are critical to lipid biosynthesis
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Acyl-CoA:diacylglycerol acyltransferase (DGAT)
|
| 650 |
|
4 |
|a functional divergence and convergence
|
| 650 |
|
4 |
|a lipids
|
| 650 |
|
4 |
|a lysophosphatidic acyltransferase (LPAAT)
|
| 650 |
|
4 |
|a microalgae
|
| 650 |
|
4 |
|a triacylglycerol biosynthesis
|
| 650 |
|
7 |
|a Diglycerides
|2 NLM
|
| 650 |
|
7 |
|a Triglycerides
|2 NLM
|
| 650 |
|
7 |
|a Acyltransferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.3.-
|2 NLM
|
| 650 |
|
7 |
|a Diacylglycerol O-Acyltransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.3.1.20
|2 NLM
|
| 700 |
1 |
|
|a Wu, Xiaoying
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wei, Ziwang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhao, Liang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Zhongze
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Liang, Qing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zheng, Jie
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Yu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Li, Yanhua
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Huang, Linfei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hu, Qiang
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Han, Danxiang
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 72(2021), 2 vom: 02. Feb., Seite 510-524
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:72
|g year:2021
|g number:2
|g day:02
|g month:02
|g pages:510-524
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/eraa451
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 72
|j 2021
|e 2
|b 02
|c 02
|h 510-524
|