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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.16973
|2 doi
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|a pubmed24n1052.xml
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|a (DE-627)NLM315615869
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|a (NLM)32990949
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Liu, Miaoxia
|e verfasserin
|4 aut
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| 245 |
1 |
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|a CERBERUS is critical for stabilization of VAPYRIN during rhizobial infection in Lotus japonicus
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 14.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.
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|a CERBERUS (also known as LIN) and VAPYRIN (VPY) are essential for infection of legumes by rhizobia and arbuscular mycorrhizal fungi (AMF). Medicago truncatula LIN (MtLIN) was reported to interact with MtVPY, but the significance of this interaction is unclear and the function of VPY in Lotus japonicus has not been studied. We demonstrate that CERBERUS has auto-ubiquitination activity in vitro and is localized within distinct motile puncta in L. japonicus root hairs and in Nicotiana benthamiana leaves. CERBERUS colocalized with the trans-Golgi network/early endosome markers. In L. japonicus, two VPY orthologs (LjVPY1 and LjVPY2) were identified. CERBERUS interacted with and colocalized with both LjVPY1 and LjVPY2. Co-expression of CERBERUS with LjVPY1 or LjVPY2 in N. benthamiana led to increased protein levels of LjVPY1 and LjVPY2, which accumulated as mobile punctate bodies in the cytoplasm. Conversely, LjVPY2 protein levels decreased in cerberus roots after rhizobial inoculation. Mutant analysis indicates that LjVPY1 and LjVPY2 are required for rhizobial infection and colonization by AMF. Our data suggest that CERBERUS stabilizes LjVPY1 and LjVPY2 within the trans-Golgi network/early endosome, where they might function to regulate endocytic trafficking and/or the formation or recycling of signaling complexes during rhizobial and AMF symbiosis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a CERBERUS
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4 |
|a TGN/EE vesicles
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|a VAPYRIN
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| 650 |
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|a nodulation
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|a stabilization
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| 650 |
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|a ubiquitination
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Jia, Ning
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Xiaolin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Ruijun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Qi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murray, Jeremy D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Downie, J Allan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Fang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 229(2021), 3 vom: 29. Feb., Seite 1684-1700
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:229
|g year:2021
|g number:3
|g day:29
|g month:02
|g pages:1684-1700
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| 856 |
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|u http://dx.doi.org/10.1111/nph.16973
|3 Volltext
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