Meta-topolin stimulates de novo shoot organogenesis and plant regeneration in cassava

© The Author(s) 2017.

Bibliographische Detailangaben
Veröffentlicht in:Plant cell, tissue and organ culture. - 1987. - 132(2018) vom: 01., Seite 219-224
1. Verfasser: Chauhan, Raj Deepika (VerfasserIn)
Weitere Verfasser: Taylor, Nigel James
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Plant cell, tissue and organ culture
Schlagworte:Journal Article Cassava Meta-topolin Organogenesis Shoot regeneration
Beschreibung
Zusammenfassung:© The Author(s) 2017.
A novel protocol for de novo shoot organogenesis from cassava has been developed utilizing meta-topolin to stimulate shoot regeneration from leaf, petiole and stem internode explants. While use of meta-topolin alone was capable of inducing shoot regeneration, a two-stage system combining meta-topolin with 2,4-D in a first stage medium, followed by subculture onto elevated levels of meta-topolin, was superior for inducing multiple shoot regeneration events in more than 35% of explants in cultivar TME 7. Caulogenesis was achieved in eleven additional cultivars. Metatopolin was also found to be beneficial for stimulating shoot regeneration from somatic embryos and cotyledon explants. The shoot organogenesis techniques described enhance the capacity of existing embryogenic systems and present previously unavailable morphogenic pathways for developing genetic transformation and gene editing technologies in cassava
Beschreibung:Date Revised 01.10.2020
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:0167-6857
DOI:10.1007/s11240-017-1315-3