Inositol-requiring enzyme 1 (IRE1) plays for AvrRpt2-triggered immunity and RIN4 cleavage in Arabidopsis under endoplasmic reticulum (ER) stress

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 156(2020) vom: 09. Nov., Seite 105-114
1. Verfasser:	Chakraborty, Rupak (VerfasserIn)
Weitere Verfasser:	Uddin, Shahab, Macoy, Donah Mary, Park, Si On, Van Anh, Duong Thu, Ryu, Gyeong Ryul, Kim, Young Hun, Lee, Jong-Yeol, Cha, Joon-Yung, Kim, Woe-Yeon, Lee, Sang Yeol, Kim, Min Gab
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Bacterial growth ER stress IRE1 RIN4 Tunicamycin UPR Arabidopsis Proteins Bacterial Proteins Intracellular Signaling Peptides and Proteins mehr... Pathogen-Associated Molecular Pattern Molecules RIN4 protein, Arabidopsis avrRpt2 protein, Pseudomonas syringae Inositol 4L6452S749 Protein Serine-Threonine Kinases EC 2.7.11.1

Beschreibung
Zusammenfassung:	Copyright © 2020. Published by Elsevier Masson SAS. Many stresses induce the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum, a phenomenon known as ER stress. In response to ER stress, cells initiate a protective response, known as unfolded protein response (UPR), to maintain cellular homeostasis. The UPR sensor, inositol-requiring enzyme 1 (IRE1), catalyzes the cytoplasmic splicing of bZIP transcription factor-encoding mRNAs to activate the UPR signaling pathway. Recently, we reported that pretreatment of Arabidopsis thaliana plants with tunicamycin, an ER stress inducer, increased their susceptibility to bacterial pathogens; on the other hand, IRE1 deficient mutants were susceptible to Pseudomonas syringae pv. maculicola (Psm) and failed to induce salicylic acid (SA)-mediated systemic acquired resistance. However, the functional relationship of IRE1 with the pathogen and TM treatment remains unknown. In the present study, we showed that bacterial pathogen-associated molecular patterns (PAMPs) induced IRE1 expression; however, PAMP-triggered immunity (PTI) response such as callose deposition, PR1 protein accumulation, or Pst DC3000 hrcC growth was not altered in ire1 mutants. We observed that IRE1 enhanced plant immunity against the bacterial pathogen P. syringae pv. tomato DC3000 (Pst DC3000) under ER stress. Moreover, TM-pretreated ire1 mutants were more susceptible to the avirulent strain Pst DC3000 (AvrRpt2) and showed greater cell death than wild-type plants during effector-triggered immunity (ETI). Additionally, Pst DC3000 (AvrRpt2)-mediated RIN4 degradation was reduced in ire1 mutants under TM-induced ER stress. Collectively, our results reveal that IRE1 plays a pivotal role in the immune signaling pathway to activate plant immunity against virulent and avirulent bacterial strains under ER stress
Beschreibung:	Date Completed 13.01.2021 Date Revised 04.12.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2020.09.002