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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-04-20-0816-RE
|2 doi
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|a pubmed24n1049.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Ye, Weimin
|e verfasserin
|4 aut
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|a Molecular Characterization of an Emerging Root-Knot Nematode Meloidogyne enterolobii in North Carolina, USA
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.04.2021
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|a Date Revised 23.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a An emerging threat to agriculture, Meloidogyne enterolobii Yang & Eisenback, 1983, is a tropical species and considered to be the most damaging root-knot nematode (RKN) in the world because of its wide host range, aggressiveness, and ability to overcome resistance to RKN in many crops. It was first detected in the United States on ornamental plants in Florida in 2001 but has since been identified in North Carolina, South Carolina, and Louisiana. Several thousand RKN populations were collected from North Carolina field crops, ornamental plants, and turfgrasses for species identification in the Nematode Assay Laboratory in the North Carolina Department of Agriculture & Consumer Services. From 2006 to 2019, root systems showing galling symptoms were dissected under the microscope, and females were obtained for DNA analysis. When only soil samples were submitted, the second-stage juveniles or males were used instead. Molecular characterization was performed via polymerase chain reaction with species-specific primers and DNA sequencing on the ribosomal DNA 18S-ITS1-5.8S and 28S D2/D3 and mitochondrial DNA CoxII-16S. One hundred thirty-five representative RKN populations from North Carolina were characterized and identified as M. enterolobii. Six populations from China where the species was originally described were included in this study for identity confirmation and comparison. As of December 2019, M. enterolobii has been confirmed from a limited number of fields in 11 North Carolina counties: Columbus, Craven, Greene, Harnett, Johnston, Lenoir, Nash, Pitt, Sampson, Wayne, and Wilson. Currently, M. enterolobii is the most important emerging RKN species in the United States and causes severe damage to agronomic and horticultural crops, especially sweetpotato in North Carolina
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|a Journal Article
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|a 28S D2/D3
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|a DNA sequencing
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|a field crops
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|a internal transcribed spacer 1
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|a mitochondrial DNA cytochrome oxidase gene subunit II-16S
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|a molecular diagnosis
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|a nematodes
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|a pathogen detection
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|a rDNA 18S
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|a Koenning, Steve R
|e verfasserin
|4 aut
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|a Zeng, Yongsan
|e verfasserin
|4 aut
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|a Zhuo, Kan
|e verfasserin
|4 aut
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|a Liao, Jinling
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant disease
|d 1997
|g 105(2021), 4 vom: 10. Apr., Seite 819-831
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|x 0191-2917
|7 nnns
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|g volume:105
|g year:2021
|g number:4
|g day:10
|g month:04
|g pages:819-831
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|u http://dx.doi.org/10.1094/PDIS-04-20-0816-RE
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