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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1111/nph.16922
|2 doi
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|a pubmed24n1049.xml
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|a (DE-627)NLM314826831
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|a (NLM)32910470
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Heck, Melanie A
|e verfasserin
|4 aut
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|a Axenic in vitro cultivation of 19 peat moss (Sphagnum L.) species as a resource for basic biology, biotechnology, and paludiculture
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|c 2021
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 14.05.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a ©2020 The Authors New Phytologist ©2020 New Phytologist Trust.
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|a Sphagnum farming can substitute peat with renewable biomass and thus help mitigate climate change. Large volumes of the required founder material can only be supplied sustainably by axenic cultivation in bioreactors. We established axenic in vitro cultures from sporophytes of 19 Sphagnum species collected in Austria, Germany, Latvia, the Netherlands, Russia, and Sweden: S. angustifolium, S. balticum, S. capillifolium, S. centrale, S. compactum, S. cuspidatum, S. fallax, S. fimbriatum, S. fuscum, S. lindbergii, S. medium/divinum, S. palustre, S. papillosum, S. rubellum, S. russowii, S. squarrosum, S. subnitens, S. subfulvum and S. warnstorfii. These species cover five of the six European Sphagnum subgenera; namely, Acutifolia, Cuspidata, Rigida, Sphagnum and Squarrosa. Their growth was measured in suspension cultures, whereas their ploidy was determined by flow cytometry and compared with the genome size of Physcomitrella patens. We identified haploid and diploid Sphagnum species, found that their cells are predominantly arrested in the G1 phase of the cell cycle, and did not find a correlation between plant productivity and ploidy. DNA barcoding was achieved by sequencing introns of the BRK1 genes. With this collection, high-quality founder material for diverse large-scale applications, but also for basic Sphagnum research, is available from the International Moss Stock Center
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Sphagnum magellanicum
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|a DNA barcoding
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|a Sphagnum farming
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|a cell cycle arrest
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|a climate change
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|a genome size
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|a peat moss
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|a peatland restoration
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|a Lüth, Volker M
|e verfasserin
|4 aut
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|a van Gessel, Nico
|e verfasserin
|4 aut
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|a Krebs, Matthias
|e verfasserin
|4 aut
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|a Kohl, Mira
|e verfasserin
|4 aut
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|a Prager, Anja
|e verfasserin
|4 aut
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|a Joosten, Hans
|e verfasserin
|4 aut
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|a Decker, Eva L
|e verfasserin
|4 aut
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|a Reski, Ralf
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 229(2021), 2 vom: 01. Jan., Seite 861-876
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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|g volume:229
|g year:2021
|g number:2
|g day:01
|g month:01
|g pages:861-876
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|u http://dx.doi.org/10.1111/nph.16922
|3 Volltext
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