The moso bamboo drought-induced 19 protein PheDi19-8 functions oppositely to its interacting partner, PheCDPK22, to modulate drought stress tolerance
Copyright © 2020. Published by Elsevier B.V.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 299(2020) vom: 01. Okt., Seite 110605 |
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| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article DREB2A Drought stress Moso bamboo PheCDPK22 PheDi19-8 Protein interaction Plant Proteins |
| Zusammenfassung: | Copyright © 2020. Published by Elsevier B.V. Drought-induced 19 (Di19) proteins play crucial roles in regulating stress responses, but the exact mechanisms underlying their involvement in moso bamboo are not fully understood. In this study, PheDi19-8 of moso bamboo (Phyllostachys edulis) was isolated and characterized. PheDi19-8 was a nuclear protein and has a high expression under various abiotic stresses, including drought and salt. As revealed by phenotypic and physiological analyses, ectopic overexpression of PheDi19-8 in Arabidopsis and rice enhanced drought tolerance. Under drought stress, the PheDi19-8-overexpressing lines showed smaller stomatal apertures and higher survival rate in comparison to the wild-type plants, as well as the PheDi19-8-overexpressing lines had higher biomass and souble sugar, but lower relative electrolyte leakage and malondialdehyde. Further investigation revealed that PheDi19-8 interacted with PheCDPK22, and their interaction decreased the DNA-binding activity of PheDi19-8. However, overexpression of PheCDPK22 enhanced Arabidopsis sensitivity to drought stress. Moreover, the expression of marker genes, including LEA, RD22, DREB2A and RD29A, was up-regulated in the PheDi19-8-overexpressing lines but down-regulated in the PheCDPK22-overexpressing. Further yeast one-hybrid and EMSA assays indicated that PheDi19-8 directly binds to the promoter of DREB2A. These results provided new insight into the interaction of PheCDPK22 and PheDi19-8 that functions oppositely to regulate drought stress in plants |
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| Beschreibung: | Date Completed 01.03.2021 Date Revised 01.03.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2020.110605 |