Current status and future opportunities for serial crystallography at MAX IV Laboratory

Current status and future opportunities for serial crystallography at MAX IV Laboratory

open access.

Bibliographische Detailangaben
Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 27(2020), Pt 5 vom: 01. Sept., Seite 1095-1102
1. Verfasser: Shilova, Anastasya (VerfasserIn)
Weitere Verfasser: Lebrette, Hugo, Aurelius, Oskar, Nan, Jie, Welin, Martin, Kovacic, Rebeka, Ghosh, Swagatha, Safari, Cecilia, Friel, Ross J, Milas, Mirko, Matej, Zdenek, Högbom, Martin, Brändén, Gisela, Kloos, Marco, Shoeman, Robert L, Doak, Bruce, Ursby, Thomas, Håkansson, Maria, Logan, Derek T, Mueller, Uwe
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article high-viscosity injectors macromolecular crystallography sample delivery serial crystallography Silicon Compounds silicon nitride QHB8T06IDK
Beschreibung
Zusammenfassung:open access.
Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed
Beschreibung:Date Completed 25.06.2021
Date Revised 10.10.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1600-5775
DOI:10.1107/S1600577520008735