Reduced serum methods for contact-based coculture of human dermal fibroblasts and epidermal keratinocytes

Direct contact-based coculture of human dermal fibroblasts and epidermal keratinocytes has been a long-standing and challenging issue owing to different serum and growth factor requirements of the two cell types. Existing protocols employ high serum concentrations (up to 10% fetal bovine serum), com...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 69(2020), 5 vom: 03. Nov., Seite 347-355
1. Verfasser: Kadam, Snehal (VerfasserIn)
Weitere Verfasser: Vandana, Madhusoodhanan, Kaushik, Karishma S
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't coculture fetal bovine serum fibroblasts keratinocytes reduced serum wound bed Culture Media