Phylogenetic characterization of Polish isolates of infectious pancreatic necrosis virus in salmonid fish

Phylogenetic characterization of Polish isolates of infectious pancreatic necrosis virus in salmonid fish

© 2020 John Wiley & Sons Ltd.

Détails bibliographiques
Publié dans:Journal of fish diseases. - 1998. - 43(2020), 11 vom: 15. Nov., Seite 1443-1451
Auteur principal: Maj-Paluch, Joanna (Auteur)
Autres auteurs: Matras, Marek, Borzym, Ewa, Stachnik, Magdalena, Reichert, Michal
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:Journal of fish diseases
Sujets:Journal Article genogroup infectious pancreatic necrosis virus phylogenetic tree
Description
Résumé:© 2020 John Wiley & Sons Ltd.
INTRODUCTION: Infectious pancreatic necrosis virus belongs to the genus Aquabirnavirus and family Birnaviridae. By VP2 gene similarity, aquatic birnavirus is clustered into seven genogroups. The aim of this study was to genetically analyse IPN viruses occurring on Polish fish farms
MATERIALS AND METHODS: Samples from freshwater fish mostly from 2012 to 2013 and from northern Poland were examined for the presence of IPN virus using isolation on cell cultures, real-time RT-PCR and RT-PCR. Fragments of 1,377 and 1,079 bp of the VP2 and VP5 genes, respectively, were sequenced, and the results were assembled into one consensus and analysed by Geneious software. The same VP2 gene region was compared and a phylogenetic tree generated by the neighbour-joining method and MEGA6 software
RESULTS: All tested Polish isolates belonged to genogroup 5, like other European Spajurup isolates
CONCLUSION: Our findings prove that there is only one IPN virus genogroup in Poland. Polish isolates show close relationships with each other. There is a close relationship between Polish isolates and isolates from Turkey, Spain and Iran. Isolate 57 is a separate branch related to isolates from the United States and Taiwan. This points to the likelihood of past virus introduction via import of stock from those countries
Description:Date Completed 31.03.2021
Date Revised 31.03.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1365-2761
DOI:10.1111/jfd.13249