Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia

Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Bačka region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out o...

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Veröffentlicht in:Plant disease. - 1997. - 105(2021), 4 vom: 25. Apr., Seite 1080-1090
1. Verfasser: Marković, Sanja (VerfasserIn)
Weitere Verfasser: Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Kosovac, Andrea, Poštić, Dobrivoj, Popović, Tatjana
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article Dickeya MLSA Pectobacterium characterization disease development and spread epidemiology pathogen detection potato prokaryotes yield loss and economic impacts
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520 |a Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Bačka region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia 
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650 4 |a Dickeya 
650 4 |a MLSA 
650 4 |a Pectobacterium 
650 4 |a characterization 
650 4 |a disease development and spread 
650 4 |a epidemiology 
650 4 |a pathogen detection 
650 4 |a potato 
650 4 |a prokaryotes 
650 4 |a yield loss and economic impacts 
700 1 |a Stanković, Slaviša  |e verfasserin  |4 aut 
700 1 |a Jelušić, Aleksandra  |e verfasserin  |4 aut 
700 1 |a Iličić, Renata  |e verfasserin  |4 aut 
700 1 |a Kosovac, Andrea  |e verfasserin  |4 aut 
700 1 |a Poštić, Dobrivoj  |e verfasserin  |4 aut 
700 1 |a Popović, Tatjana  |e verfasserin  |4 aut 
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