Direct measurement of deubiquitinating enzyme activity in intact cells using a protease-resistant, cell-permeable, peptide-based reporter
Deubiquitinating enzymes (DUBs) regulate the removal of the polyubiquitin chain from proteins targeted for degradation. Current approaches to quantify DUB activity are limited to test tube-based assays that incorporate enzymes or cell lysates, but not intact cells. The goal of this work was to devel...
| Publié dans: | Biochemical engineering journal. - 1998. - 151(2019) vom: 15. Nov. |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2019
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| Accès à la collection: | Biochemical engineering journal |
| Sujets: | Journal Article Cell penetrating peptides Deubiquitinating enzymes (DUBs) Fluorometry Peptide-based biosensors Protease-resilient peptides Single cell analysis |
| Accès en ligne |
Volltext |