Direct measurement of deubiquitinating enzyme activity in intact cells using a protease-resistant, cell-permeable, peptide-based reporter

Deubiquitinating enzymes (DUBs) regulate the removal of the polyubiquitin chain from proteins targeted for degradation. Current approaches to quantify DUB activity are limited to test tube-based assays that incorporate enzymes or cell lysates, but not intact cells. The goal of this work was to devel...

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Bibliographische Detailangaben

Veröffentlicht in:	Biochemical engineering journal. - 1998. - 151(2019) vom: 15. Nov.
1. Verfasser:	Safa, Nora (VerfasserIn)
Weitere Verfasser:	Pettigrew, Jacob H, Gauthier, Ted J, Melvin, Adam T
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	Biochemical engineering journal
Schlagworte:	Journal Article Cell penetrating peptides Deubiquitinating enzymes (DUBs) Fluorometry Peptide-based biosensors Protease-resilient peptides Single cell analysis