Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening

Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening

Copyright © 2020 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 155(2020) vom: 25. Okt., Seite 429-436
1. Verfasser: Wang, Baoxiang (VerfasserIn)
Weitere Verfasser: Zhang, Manman, Zhang, Jie, Huang, Liping, Chen, Xi, Jiang, Mingyi, Tan, Mingpu
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article BAX inhibitor Cd tolerance HMAD isoprenylated Protein NAC transcription Factor Rapid ALkalinization factors Rice Cadmium 00BH33GNGH
Beschreibung
Zusammenfassung:Copyright © 2020 Elsevier Masson SAS. All rights reserved.
The bioaccumulation of cadmium (Cd) in crop and the subsequent food chain has aroused extensive concerns. However, the underlying molecular mechanisms of plant Cd tolerance remain to be clarified from the viewpoint of novel candidate genes. Here we described a highly efficient approach for preliminary identifying rice Cd-tolerant genes through the yeast-based cDNA library survival screening combined with high-throughput sequencing strategy. About 690 gene isoforms were identified as being Cd-tolerant candidates using this shotgun approach. Among the Cd-tolerant genes identified, several categories of genes such as BAX inhibitor (BI), NAC transcription factors and Rapid ALkalinization Factors (RALFs) were of particular interest, and their function of Cd tolerance was further validated via heterologous expression, which suggested that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves. Regarding the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated domain (HMAD) isoprenylated protein HIPP42 was particularly noteworthy. Further elucidation of these genes associated with Cd tolerance in rice will benefit agricultural activities
Beschreibung:Date Completed 17.12.2020
Date Revised 17.12.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2020.07.046