Genome-wide functional analysis of hot pepper immune receptors reveals an autonomous NLR clade in seed plants

Genome-wide functional analysis of hot pepper immune receptors reveals an autonomous NLR clade in seed plants

© 2020 The Authors New Phytologist © 2020 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 229(2021), 1 vom: 01. Jan., Seite 532-547
1. Verfasser: Lee, Hye-Young (VerfasserIn)
Weitere Verfasser: Mang, Hyunggon, Choi, Eunhye, Seo, Ye-Eun, Kim, Myung-Shin, Oh, Soohyun, Kim, Saet-Byul, Choi, Doil
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Capsicum annuum cell death coiled-coil domain plant NLR singleton NLR NLR Proteins Plant Proteins
Beschreibung
Zusammenfassung:© 2020 The Authors New Phytologist © 2020 New Phytologist Trust.
Plants possess hundreds of intracellular immune receptors encoding nucleotide-binding domain leucine-rich repeat (NLR) proteins. Full-length NLRs or a specific domain of NLRs often induce plant cell death in the absence of pathogen infection. In this study we used genome-wide transient expression analysis to identify a group of NLRs (ANLs; ancient and autonomous NLRs) carrying autoactive coiled-coil (CCA ) domains in pepper (Capsicum annuum). CCA -mediated cell death mimics hypersensitive cell death triggered by the interaction between NLRs and pathogen effectors. Sequence alignment and mutagenesis analyses revealed that the intact α1 helix of CCA s is critical for both CCA - and ANL-mediated cell death. Cell death induced by CCA s does not require NRG1/ADR1 or NRC type helper NLRs, suggesting ANLs may function as singleton NLRs. We also found that CCA s localize to the plasma membrane, as demonstrated for Arabidopsis singleton NLR ZAR1. Extended studies revealed that autoactive CCA s are well conserved in other Solanaceae plants as well as in rice, a monocot plant. Further phylogenetic analyses revealed that ANLs are present in all tested seed plants (spermatophytes). Our study not only uncovers the autonomous NLR clade in plants but also provides powerful resources for dissecting the underlying molecular mechanism of NLR-mediated cell death in plants
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16878