A calmodulin-like CmCML13 from Cucumis melo improved transgenic Arabidopsis salt tolerance through reduced shoot's Na+, and also improved drought resistance

A calmodulin-like CmCML13 from Cucumis melo improved transgenic Arabidopsis salt tolerance through reduced shoot's Na+, and also improved drought resistance

Copyright © 2020 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 155(2020) vom: 11. Okt., Seite 271-283
1. Verfasser: Yang, Senlin (VerfasserIn)
Weitere Verfasser: Xiong, Xue, Arif, Samiah, Gao, Liwei, Zhao, Lina, Shah, Iftikhar Hussain, Zhang, Yidong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article CmCML13 (calmodulin-like 13) Drought stress HKT1 (high affinity K(+) transporter) Melon (Cucumis melo L) Salt stress Calmodulin Plant Proteins
Beschreibung
Zusammenfassung:Copyright © 2020 Elsevier Masson SAS. All rights reserved.
The calmodulin-like proteins (CMLs) are a large family involved in plant biological processes. A calmodulin-like gene CmCML13 (GenBank accession number: MT340534) from melon (Cucumis melo L.) was isolated and functionally analyzed. CmCML13 was predicted to possess 3 EF-hands in which only the first EF-hand could bind with Ca2+. Subcellular localization assay revealed that CmCML13 was localized in nucleus, cell membrane, vacuolar membrane and cytoplasmic strand. The transcript level of CmCML13 was temporally and spatially regulated under salt stress. Constitutive expression of CmCML13 in the Arabidopsis thaliana enhanced salt tolerance at seeds germination. CmCML13 improved the transgenic Arabidopsis plants salt tolerance by significantly reducing Na+ content of shoots, which was unrelated to HKT1-involving pathway. Moreover, overexpressing of CmCML13 in Arabidopsis showed stronger drought tolerance. This study demonstrates that the CmCML13 is an important multifunctional protein associated with salt and drought stress, which may play a key role in stress signaling pathway
Beschreibung:Date Completed 17.12.2020
Date Revised 17.12.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2020.07.013