Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin

Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a. Initially, the endolysin was cloned using BamHI/XhoI, resulti...

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Veröffentlicht in:	BioTechniques. - 1988. - 69(2020), 3 vom: 16. Sept., Seite 161-170
1. Verfasser:	Tham, Hong Y (VerfasserIn)
Weitere Verfasser:	Song, Adelene A-L, Yusoff, Khatijah, Tan, Geok H
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't MRSA antimicrobial activity endolysin inclusion body pET28a protein expression Recombinant Proteins Endopeptidases mehr... EC 3.4.- EC 3.4.99.-