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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.0c01941
|2 doi
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|a pubmed25n1045.xml
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|a eng
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|a da Rocha Rodrigues, Rebeca
|e verfasserin
|4 aut
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|a Langmuir and Langmuir-Blodgett Films of Poly[(9,9-dioctylfluorene)-co-(3-hexylthiophene)] for Immobilization of Phytase
|b Possible Application as a Phytic Acid Sensor
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 06.10.2020
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|a Date Revised 06.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a In this work, the copolymer poly[(9,9-dioctylfluorene)-co-(3-hexylthiophene)] was employed as a matrix for immobilizing phytase, aiming at the detection of phytic acid. The copolymer was spread on the air-water interface forming Langmuir monolayers and phytase adsorbed from the aqueous subphase. The interactions between the copolymer and the enzyme components were investigated with surface pressure and surface potential-area isotherms, Brewster angle microscopy, and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The enzyme could be incorporated in the monolayers from the aqueous subphase, expanding the copolymer films and maintaining its secondary structure. The polymeric films presented a morphological heterogeneous pattern at the air-water interface because of the ability of their chains to fold and entangle, causing inherent defects in the organization as well as unbalanced lateral distribution at the air-water interface because of the formation of aggregates. The interfacial films were transferred to solid supports as Langmuir-Blodgett films and characterized by PM-IRRAS and scanning electronic microscopy, which showed not only the co-transfer of the enzyme but also the maintenance of their heterogeneous morphological pattern. The enzymatic activity of the blended film was analyzed by UV-vis spectroscopy and allowed the estimation of the value of the Michaelis constant (13.08 mM), demonstrating the feasibility of the system to selectively detect phytic acid for biosensing purposes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Caseli, Luciano
|e verfasserin
|4 aut
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|a Péres, Laura Oliveira
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 36(2020), 35 vom: 08. Sept., Seite 10587-10596
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
1 |
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|g volume:36
|g year:2020
|g number:35
|g day:08
|g month:09
|g pages:10587-10596
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|u http://dx.doi.org/10.1021/acs.langmuir.0c01941
|3 Volltext
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