Optimal temporal-spatial fluorescence techniques for phenotyping nitrogen status in oilseed rape

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Détails bibliographiques
Publié dans:Journal of experimental botany. - 1985. - 71(2020), 20 vom: 22. Okt., Seite 6429-6443
Auteur principal: Sun, Dawei (Auteur)
Autres auteurs: Xu, Haixia, Weng, Haiyong, Zhou, Weijun, Liang, Yan, Dong, Xiaoya, He, Yong, Cen, Haiyan
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:Journal of experimental botany
Sujets:Journal Article Research Support, Non-U.S. Gov't Chlorophyll fluorescence OJIP transients multicolor fluorescence imaging nitrogen oilseed rape photosynthesis pulse amplitude-modulated chlorophyll fluorescence imaging Chlorophyll plus... 1406-65-1 Nitrogen N762921K75
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520 |a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. 
520 |a Nitrogen (N) fertilizer maximizes the growth of oilseed rape (Brassica napus L.) by improving photosynthetic performance. Elucidating the dynamic relationship between fluorescence and plant N status could provide a non-destructive diagnosis of N status and the breeding of N-efficient cultivars. The aim of this study was to explore the impacts of different N treatments on photosynthesis at a spatial-temporal scale and to evaluate the performance of three fluorescence techniques for the diagnosis of N status. One-way ANOVA and linear discriminant analysis were applied to analyze fluorescence data acquired by a continuous excitation chlorophyll fluorimeter (OJIP transient analysis), pulse amplitude-modulated chlorophyll fluorescence (PAM-ChlF), and multicolor fluorescence (MCF) imaging. The results showed that the maximum quantum efficiency of PSII photochemistry (Fv/Fm) and performance index for photosynthesis (PIABS) of bottom leaves were sensitive to N status at the bolting stage, whereas the red fluorescence/far-red fluorescence ratio of top leaves was sensitive at the early seedling stage. Although the classification of N treatments by the three techniques achieved comparable accuracies, MCF imaging showed the best potential for early diagnosis of N status in field phenotyping because it had the highest sensitivity in the top leaves, at the early seedling stage. The findings of this study could facilitate research on N management and the breeding of N-efficient cultivars 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Chlorophyll fluorescence 
650 4 |a OJIP transients 
650 4 |a multicolor fluorescence imaging 
650 4 |a nitrogen 
650 4 |a oilseed rape 
650 4 |a photosynthesis 
650 4 |a pulse amplitude-modulated chlorophyll fluorescence imaging 
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650 7 |a 1406-65-1  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
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700 1 |a Xu, Haixia  |e verfasserin  |4 aut 
700 1 |a Weng, Haiyong  |e verfasserin  |4 aut 
700 1 |a Zhou, Weijun  |e verfasserin  |4 aut 
700 1 |a Liang, Yan  |e verfasserin  |4 aut 
700 1 |a Dong, Xiaoya  |e verfasserin  |4 aut 
700 1 |a He, Yong  |e verfasserin  |4 aut 
700 1 |a Cen, Haiyan  |e verfasserin  |4 aut 
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