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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13178
|2 doi
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|a pubmed25n1043.xml
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Wang, Sijia
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Molecular and light microscopy evidence for the transfer of Myxobolus honghuensis from Carassius auratus gibelio broodfish to progeny
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 18.02.2021
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|a Date Revised 18.02.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2020 John Wiley & Sons Ltd.
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|a Myxozoans usually have a complex life cycle involving indirect transmission between vertebrate and invertebrate hosts. The vertical transmission of these parasites in vertebrate hosts has not been documented so far. Here, we assessed whether the myxozoan parasite Myxobolus honghuensis is vertically transmitted in naturally infected allogynogenetic gibel carp Carassius auratus gibelio (Bloch). M. honghuensis infection of broodfish, fertilized eggs and laboratory-cultured progeny was monitored in 2018 and 2019. The presporogonic stage was microscopically observed in the pharynx of broodfish and their progeny. In situ hybridization confirmed the presence of M. honghuensis presporogonic stage in the pharynx of broodfish and progeny. Nested PCR results showed that M. honghuensis was present in tissues and eggs of broodfish, fertilized eggs and their corresponding progeny. The sequences obtained from broodfish and progeny showed 98.0-99.8% similarity with ITS-5.8S rDNA of M. honghuensis. This study provides molecular and light microscopy evidence for the transfer of M. honghuensis from broodfish to progeny via the eggs, but it is insufficient to assert that M. honghuensis can transmit vertically in naturally infected allogynogenetic gibel carp. This is the first record about vertical transfer of myxozoan in the vertebrate host
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|a Journal Article
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|a Myxozoans
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| 650 |
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|a allogynogenetic gibel carp
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| 650 |
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4 |
|a in situ hybridization
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| 650 |
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4 |
|a nested PCR
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| 650 |
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7 |
|a RNA, Ribosomal, 5.8S
|2 NLM
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| 700 |
1 |
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|a Zhang, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Qingxiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhai, Yanhua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gu, Zemao
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 43(2020), 10 vom: 01. Okt., Seite 1177-1184
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnas
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| 773 |
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|g volume:43
|g year:2020
|g number:10
|g day:01
|g month:10
|g pages:1177-1184
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|u http://dx.doi.org/10.1111/jfd.13178
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