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20231225145840.0 |
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cr uuu---uuuuu |
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231225s2021 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-04-20-0732-RE
|2 doi
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|a pubmed24n1043.xml
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|a (NLM)32734845
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Nguyen-Huu, Trong
|e verfasserin
|4 aut
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| 245 |
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|a Development of a DNA-Based Real-Time PCR Assay To Quantify Allorhizobium vitis Over Time in Grapevine (Vitis vinifera L.) Plantlets
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| 264 |
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|c 2021
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.02.2021
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|a Date Revised 26.02.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Allorhizobium vitis is the primary causal pathogen of grapevine crown gall disease. Because this endophytic bacterium can survive as a systemic latent (symptomless) infection in grapevine, detecting and monitoring its development in planta is of great importance. In plant bacteria studies, plate counting is routinely used as a simple and reliable method to evaluate the bacterial population level in planta. However, isolation techniques are time-consuming and present some disadvantages such as the risk of contamination and the need for fresh samples for research. In this study, we developed a DNA-based real-time PCR assay that can replace the classical method to monitor the development of Allorhizobium vitis in grapevine plantlets. Primers targeting Allorhizobium vitis chromosomic genes and the virulent tumor-inducing plasmid were validated. The proposed quantitative real-time PCR technique is highly reliable and reproducible to assess Allorhizobium vitis numeration at the earliest stage of infection until tumor development in grapevine plantlets. Moreover, this low-cost technique provides rapid and robust in planta quantification of the pathogen and is suitable for fundamental research to monitor bacterial development over time
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| 650 |
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4 |
|a Journal Article
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4 |
|a Agrobacterium
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| 650 |
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4 |
|a Allorhizobium vitis
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| 650 |
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4 |
|a grapevine shoots
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| 650 |
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4 |
|a pathogen detection
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| 650 |
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4 |
|a real-time PCR
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Doré, Jeanne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aït Barka, Essaïd
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lavire, Céline
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Clément, Christophe
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vial, Ludovic
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sanchez, Lisa
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 105(2021), 2 vom: 01. Feb., Seite 384-391
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:105
|g year:2021
|g number:2
|g day:01
|g month:02
|g pages:384-391
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| 856 |
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|u http://dx.doi.org/10.1094/PDIS-04-20-0732-RE
|3 Volltext
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