The MYB transcription factor TaPHR3-A1 is involved in phosphate signaling and governs yield-related traits in bread wheat

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 71(2020), 19 vom: 07. Okt., Seite 5808-5822
1. Verfasser:	Zheng, Xingwei (VerfasserIn)
Weitere Verfasser:	Liu, Cheng, Qiao, Ling, Zhao, Jiajia, Han, Ran, Wang, Xiaolu, Ge, Chuan, Zhang, Wenyun, Zhang, Shuwei, Qiao, Linyi, Zheng, Jun, Hao, Chenyang
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't TaPHR3-A1 Molecular marker Pi tolerance transgenic line wheat Phosphates Plant Proteins Proto-Oncogene Proteins c-myb Transcription Factors

Beschreibung
Zusammenfassung:	© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Improved inorganic phosphate (Pi) use efficiency in crops will be important for sustainable agriculture. Exploring molecular mechanisms that regulate Pi uptake could provide useful information for breeding wheat with improved Pi use efficiency. Here, a TaPHR3-A1 (Gene ID: TraesCS7A02G415800) ortholog of rice OsPHR3 that functions in transcriptional regulation of Pi signaling was cloned from wheat chromosome 7A. Ectopic expression of TaPHR3-A1 in Arabidopsis and rice produced enhanced vegetative growth and more seeds. Overexpression in transgenic rice led to increased biomass, grain number, and primary panicle branching by 61.23, 42.12, and 36.34% compared with the wild type. Transgenic wheat lines with down-regulation of TaPHR3-A1 exhibited retarded growth and root hair development at the seedling stage, and showed yield-related effects at the adult stage when grown in both low- and sufficient Pi conditions, indicating that TaPHR3-A1 positively regulated tolerance to low Pi. Introgression lines further confirmed the effect of TaPHR3-A1 in improving grain number. The Chinese wheat mini core collection and a recombinant inbred line analysis demonstrated that the favorable allele TaPHR3-A1-A associated with higher grain number was positively selected in breeding. A TaPHR3-A1-derived cleaved amplified polymorphic sequence marker effectively identified haplotype TaPHR3-A1-A. Our results suggested that TaPHR3-A1 was a functional regulatory factor for Pi uptake and provided useful information for marker-assisted selection for high yield in wheat
Beschreibung:	Date Completed 14.05.2021 Date Revised 31.05.2022 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/eraa355