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DE-627 |
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20231225145446.0 |
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cr uuu---uuuuu |
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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-11-19-2455-RE
|2 doi
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| 028 |
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|a pubmed24n1043.xml
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|a (DE-627)NLM312921330
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|a (NLM)32716275
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Xing, Fei
|e verfasserin
|4 aut
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| 245 |
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|a RNA-Seq Reveals Hawthorn Tree as a New Natural Host for Apple Necrotic Mosaic Virus, Possibly Associated with Hawthorn Mosaic Disease
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|c 2020
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 29.09.2020
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|a Date Revised 29.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Apple mosaic disease is widespread in the major apple-producing areas in China and is frequently associated with the presence of the newly identified Apple necrotic mosaic virus (ApNMV), belonging to subgroup 3 of Ilarvirus genus in the family of Bromoviridae. Mosaic symptoms were also observed in a hawthorn tree. Deep sequencing revealed the hawthorn tree with mosaic symptom was infected by ApNMV, which was confirmed by RT-PCR. The complete nucleotide sequences of RNA1 (3,378 nt), RNA2 (2,778 nt), and RNA3 (1,917 nt) of ApNMV from the hawthorn were obtained, sharing 93.8 to 96.8%, 89.7 to 96.1%, and 89.8 to 94.6% nucleotide identities with those from apples and crabapples, respectively. Two hypervariable regions were found, which showed 59.2 to 85.7% and 64.0 to 89.3% sequence identities at position 142 to 198 aa and at position 780 to 864 aa in the POL protein, respectively, between the hawthorn isolate and other isolates (apple, crabapple). A grafting test demonstrated that ApNMV was easily transmissible from hawthorns to apple trees, with severe chlorosis, yellowing, mosaic, curling, and necrosis. In addition, a total of 11,685 hawthorn trees were surveyed for the incidence of mosaic disease from five provinces in China, and only six were found showing typical mosaic symptoms. A total of 145 individual trees (six symptomatic, 68 asymptomatic, and 71 other symptoms) were tested for the presence or absence of ApNMV by RT-PCR. Among them, six symptomatic, four asymptomatic, and 10 other symptomatic trees tested positive for ApNMV. Taken together, these results demonstrated that the hawthorn tree was identified as a new natural host for ApNMV with a relatively low frequency (13.8%, 20 out of 145) in the main producing areas, and it was likely to be the causal pathogen of hawthorn mosaic disease
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|a Journal Article
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|a RNA-seq
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|a apple necrotic mosaic virus
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4 |
|a hawthorn mosaic disease
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|a hawthorn tree
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|a new natural host
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1 |
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|a Hou, Wanying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Massart, Sebastien
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Dehang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Wenhui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Mengji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Zhixiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Hongqing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Shifang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 104(2020), 10 vom: 01. Okt., Seite 2713-2719
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:104
|g year:2020
|g number:10
|g day:01
|g month:10
|g pages:2713-2719
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-11-19-2455-RE
|3 Volltext
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