Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed

Copyright © 2020 by JAPANESE SOCIETY OF BREEDING.

Bibliographische Detailangaben
Veröffentlicht in:Breeding science. - 1998. - 70(2020), 3 vom: 01. Juni, Seite 265-276
1. Verfasser: Sarkar, Md Abdur Rauf (VerfasserIn)
Weitere Verfasser: Otsu, Wakana, Suzuki, Akihiro, Hashimoto, Fumio, Anai, Toyoaki, Watanabe, Satoshi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article NGS daidzein gene identification genistein isoflavone mutant line soybean
Beschreibung
Zusammenfassung:Copyright © 2020 by JAPANESE SOCIETY OF BREEDING.
Novel mutant alleles related to isoflavone content are useful for breeding programs to improve the disease resistance and nutritional content of soybean. However, identification of mutant alleles from high-density mutant libraries is expensive and time-consuming because soybean has a large, complicated genome. Here, we identified the gene responsible for increased genistein-to-daidzein ratio in seed of the mutant line F333ES017D9. For this purpose, we used a time- and cost-effective approach based on selective genotyping of a small number of F2 plants showing the mutant phenotype with nearest-neighboring-nucleotide substitution-high-resolution melting analysis markers, followed by alignment of short reads obtained by next-generation sequencing analysis with the identified locus. In the mutant line, GmCHR5 harbored a single-base deletion that caused a change in the substrate flow in the isoflavone biosynthetic pathway towards genistein. Mutated GmCHR5 was expressed at a lower level during seed development than wild-type GmCHR5. Ectopic overexpression of GmCHR5 increased the production of daidzein derivatives in both the wild-type and mutant plants. The present strategy will be useful for accelerating identification of mutant alleles responsible for traits of interest in agronomically important crops
Beschreibung:Date Revised 28.09.2020
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:1344-7610
DOI:10.1270/jsbbs.19134