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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2020.153190
|2 doi
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|a pubmed25n1041.xml
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|a (DE-627)NLM312644825
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|a (NLM)32688165
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|a (PII)S0176-1617(20)30080-8
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Zhao, Man
|e verfasserin
|4 aut
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|a Extracellular Ca2+ induces desensitized cytosolic Ca2+ rise sensitive to phospholipase C inhibitor which suppresses root growth with Ca2+ dependence
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.03.2021
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|a Date Revised 01.03.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2020 Elsevier GmbH. All rights reserved.
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|a Calcium (Ca) is an essential element for all organisms. In animal cells, the plasma membrane-localized Ca receptor CaSR coupled to a phospholipase C (PLC)-dependent signaling cascade monitors extracellular Ca2+ concentrations ([Ca2+]ext) and responds with increases in cytosolic calcium concentrations ([Ca2+]cyt). Plant roots encounter variable soil conditions, but how they sense changes in [Ca2+]ext is largely unknown. In this study, we demonstrate that increasing [Ca2+]ext evokes a transient increase in [Ca2+] in the cytosol, mitochondria, and nuclei of Arabidopsis thaliana root cells. These increases were strongly desensitized to repeat applications of [Ca2+]ext, a typical feature of receptor-mediated cellular signaling in animal and plant cells. Treatment with gadolinium (Gd3+), a CaSR activator in animal cells, induced concentration-dependent increases in [Ca2+]cyt in roots, which showed self-desensitization and cross-desensitization to [Ca2+]ext-induced increases in [Ca2+]cyt (EICC). EICC was sensitive to extracellular H+, K+, Na+, and Mg2+ levels. Treatment with the PLC inhibitor neomycin suppressed EICC and Ca accumulation in roots. The inhibitory effect of neomycin on root elongation was fully rescued by increasing [Ca2+]ext but not [Mg2+] or [K+] in the growth medium. These results suggest that [Ca2+]ext and the movement of Ca2+ into the cytosol of plant roots are regulated by a receptor-mediated signaling pathway involving PLC
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|a Journal Article
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|a Aequorin
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|a Arabidopsis
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|a Calcium
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|a Phospholipase C
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|a Receptor
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|a Plant Proteins
|2 NLM
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|a Protein Synthesis Inhibitors
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|a Aequorin
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|a 50934-79-7
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|a Type C Phospholipases
|2 NLM
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|a EC 3.1.4.-
|2 NLM
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|a Neomycin
|2 NLM
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|a I16QD7X297
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Chen, Jianhua
|e verfasserin
|4 aut
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|a Jin, Huiqing
|e verfasserin
|4 aut
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| 700 |
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|a Qi, Zhi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 252(2020) vom: 15. Sept., Seite 153190
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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| 773 |
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|g volume:252
|g year:2020
|g day:15
|g month:09
|g pages:153190
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|u http://dx.doi.org/10.1016/j.jplph.2020.153190
|3 Volltext
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