|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM312609698 |
| 003 |
DE-627 |
| 005 |
20250227151913.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1007/s11240-020-01871-w
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1041.xml
|
| 035 |
|
|
|a (DE-627)NLM312609698
|
| 035 |
|
|
|a (NLM)32684656
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Haselmair-Gosch, Christian
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 17.03.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a © The Author(s) 2020.
|
| 520 |
|
|
|a In 2017, various orange coloured petunia on the market turned out to be genetically modified (GM) without an official authorization for commercialization. Sequence analysis suggested these undeclared plants most probably originated from a plant transformation experiment performed in the 1980s. For a deeper understanding how GM petunia entered classical breeding programmes worldwide, and whether they originated from a single source or not, we undertook a molecular genetic characterization of the T-DNA integration sites in different GM petunia cultivars and breeding lines. By means of genome walking, we isolated different T-DNA sequences, which are located at the junctions between the T-DNA(s) and the petunia DNA. Based on the results obtained we conclude that there are at least two T-DNA copies of different lengths. This is supported by Southern blot analysis. For T-DNA1, the 3'-junction sequence was isolated, whereas the 5'-junction remained unclear. In contrast, for T-DNA2, the 5'-junction sequence was isolated, whereas the sequence isolated from the 3'-region consists only of T-DNA, but did not include the junction from the T-DNA to the petunia DNA. We developed primers for event-specific PCRs and screened a set of three orange GM petunia cultivars and 126 GM offspring from a commercial breeding program. We show that both T-DNA copies are present in all our tested GM petunia samples, which underpins the assumption of a single transgenic origin of the undeclared GM petunia. Most likely, the two T-DNAs are integrated in close proximity into the petunia genome
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Anthocyanin
|
| 650 |
|
4 |
|a Event-specific transgene detection
|
| 650 |
|
4 |
|a Orange flower colour
|
| 650 |
|
4 |
|a Petunia × hybrida
|
| 650 |
|
4 |
|a Transgenic plant
|
| 700 |
1 |
|
|a Nitarska, Daria
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Walliser, Benjamin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Flachowsky, Henryk
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Marinovic, Silvija
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Halbwirth, Heidi
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant cell, tissue and organ culture
|d 1987
|g 142(2020), 2 vom: 14., Seite 415-424
|w (DE-627)NLM098150995
|x 0167-6857
|7 nnas
|
| 773 |
1 |
8 |
|g volume:142
|g year:2020
|g number:2
|g day:14
|g pages:415-424
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1007/s11240-020-01871-w
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 142
|j 2020
|e 2
|b 14
|h 415-424
|