Deletion of maize RDM4 suggests a role in endosperm maturation as well as vegetative and stress-responsive growth

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 19 vom: 07. Okt., Seite 5880-5895
1. Verfasser: Jia, Shangang (VerfasserIn)
Weitere Verfasser: Yobi, Abou, Naldrett, Michael J, Alvarez, Sophie, Angelovici, Ruthie, Zhang, Chi, Holding, David R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Cold stress Opaque mutant RDM4 endosperm leaf maize zein proteins mehr... Plant Proteins RNA 63231-63-0 Zein 9010-66-6
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520 |a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. 
520 |a Opaque kernels in maize may result from mutations in many genes, such as OPAQUE-2. In this study, a maize null mutant of RNA-DIRECTED DNA METHYLATION 4 (RDM4) showed an opaque kernel phenotype, as well as plant developmental delay, male sterility, and altered response to cold stress. We found that in opaque kernels, all zein proteins were reduced and amino acid content was changed, including increased lysine. Transcriptomic and proteomic analysis confirmed the zein reduction and proteomic rebalancing of non-zein proteins, which was quantitatively and qualitatively different from opaque-2. Global transcriptional changes were found in endosperm and leaf, including many transcription factors and tissue-specific expressed genes. Furthermore, of the more than 8000 significantly differentially expressed genes in wild type in response to cold, a significant proportion (25.9% in moderate cold stress and 40.8% in near freezing stress) were not differentially expressed in response to cold in rdm4, suggesting RDM4 may participate in regulation of abiotic stress tolerance. This initial characterization of maize RDM4 provides a basis for further investigating its function in endosperm and leaf, and as a regulator of normal and stress-responsive development 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Cold stress 
650 4 |a Opaque mutant 
650 4 |a RDM4 
650 4 |a endosperm 
650 4 |a leaf 
650 4 |a maize 
650 4 |a zein proteins 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a RNA  |2 NLM 
650 7 |a 63231-63-0  |2 NLM 
650 7 |a Zein  |2 NLM 
650 7 |a 9010-66-6  |2 NLM 
700 1 |a Yobi, Abou  |e verfasserin  |4 aut 
700 1 |a Naldrett, Michael J  |e verfasserin  |4 aut 
700 1 |a Alvarez, Sophie  |e verfasserin  |4 aut 
700 1 |a Angelovici, Ruthie  |e verfasserin  |4 aut 
700 1 |a Zhang, Chi  |e verfasserin  |4 aut 
700 1 |a Holding, David R  |e verfasserin  |4 aut 
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