|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM312362897 |
| 003 |
DE-627 |
| 005 |
20250227142717.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2020 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2020.06.011
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1041.xml
|
| 035 |
|
|
|a (DE-627)NLM312362897
|
| 035 |
|
|
|a (NLM)32659648
|
| 035 |
|
|
|a (PII)S0981-9428(20)30292-8
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Garagounis, Constantine
|e verfasserin
|4 aut
|
| 245 |
1 |
2 |
|a A hairy-root transformation protocol for Trigonella foenum-graecum L. as a tool for metabolic engineering and specialised metabolite pathway elucidation
|
| 264 |
|
1 |
|c 2020
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 10.12.2020
|
| 500 |
|
|
|a Date Revised 14.12.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2020 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a The development of genetic transformation methods is critical for enabling the thorough characterization of an organism and is a key step in exploiting any species as a platform for synthetic biology and metabolic engineering approaches. In this work we describe the development of an Agrobacterium rhizogenes-mediated hairy root transformation protocol for the crop and medicinal legume fenugreek (Trigonella foenum-graecum). Fenugreek has a rich and diverse content in bioactive specialised metabolites, notably diosgenin, which is a common precursor for synthetic human hormone production. This makes fenugreek a prime target for identification and engineering of specific biosynthetic pathways for the production of triterpene and steroidal saponins, phenolics, and galactomanans. Through this transformation protocol, we identified a suitable promoter for robust transgene expression in fenugreek. Finally, we establish the proof of principle for the utility of the fenugreek system for metabolic engineering programs, by heterologous expression of known triterpene saponin biosynthesis regulators from the related legume Medicago truncatula in fenugreek hairy roots
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Agrobacterium rhizogenes transformation
|
| 650 |
|
4 |
|a Fenugreek
|
| 650 |
|
4 |
|a Hairy root
|
| 650 |
|
4 |
|a TSAR
|
| 650 |
|
4 |
|a Trigonella foenum-Graecum
|
| 650 |
|
4 |
|a Triterpene saponin activating regulator
|
| 650 |
|
7 |
|a Saponins
|2 NLM
|
| 650 |
|
7 |
|a Diosgenin
|2 NLM
|
| 650 |
|
7 |
|a K49P2K8WLX
|2 NLM
|
| 700 |
1 |
|
|a Beritza, Konstantina
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Georgopoulou, Maria-Eleni
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sonawane, Prashant
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Haralampidis, Kosmas
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Goossens, Alain
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Aharoni, Asaph
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Papadopoulou, Kalliope K
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 154(2020) vom: 01. Sept., Seite 451-462
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
|
| 773 |
1 |
8 |
|g volume:154
|g year:2020
|g day:01
|g month:09
|g pages:451-462
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2020.06.011
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 154
|j 2020
|b 01
|c 09
|h 451-462
|