Various factors affect lipopolysaccharide sensitization in cell cultures

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantifie...

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Veröffentlicht in:	BioTechniques. - 1988. - 69(2020), 2 vom: 04. Aug., Seite 126-132
1. Verfasser:	Yang, Nan (VerfasserIn)
Weitere Verfasser:	Sin, Don D, Dorscheid, Delbert R
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't assays cell culture fetal bovine serum inflammation interleukin-8 lipopolysaccharide optimization reagents mehr... sensitization CXCL8 protein, human Culture Media Interleukin-8 Lipopolysaccharides Serum Albumin, Bovine 27432CM55Q